|
| Status |
Public on Jul 01, 2021 |
| Title |
dKO_Brain_replicate_3 |
| Sample type |
RNA |
| |
|
| Source name |
Frontal cortex of brain
|
| Organism |
Rattus norvegicus |
| Characteristics |
genotype: Abcg2-/- Abcb1a-/-
gender: Male
strain: Sprague-Dawley
age: 10 weeks
animal id: 154
tissue: Frontal cortex of brain
|
| Growth protocol |
Tissues were collected from 10 week old male rats. Liver tissue was snap frozen and maintained at -80°C until RNA extraction. Brain frontal cortex and kidney tissues were stored in RNAlaterTM (ThermoFisher P/N AM7020) following the manufacturer’s instructions until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissues using TRIzolTM Reagent (ThermoFisher P/N 15596018) according to the manufacturer's recommended procedures.
|
| Label |
Biotin
|
| Label protocol |
Sense target cDNA was prepared from 125ng of total RNA using the Ambion® WT Expression Kit (Life Technologies P/N 4411973). cDNA was fragmented and biotin-labeled using the Affymetrix® GeneChip® WT Terminal Labeling Kit (Affymetrix P/N 900671).
|
| |
|
| Hybridization protocol |
Five micrograms of cDNA was hybridized for 16h at 45C on a Rat Gene 2.0 ST GeneChip array.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console v1.1 software
|
| |
|
| Submission date |
Oct 05, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
geoffrey.neale@stjude.org
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL17117 |
| Series (1) |
| GSE159058 |
Integrated metabolomic and transcriptomic analysis reveals metabolic adaptation of rats to Abcg2/Abcb1a knockout |
|
