|
| Status |
Public on May 30, 2023 |
| Title |
KO1_E13 |
| Sample type |
SRA |
| |
|
| Source name |
KO_E13
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6N
tissue: whole heart
age: E13.5
genotype: MyHC-Creld1 KO
pupid: 11685 #8
prepdate: 3/7/2016
litter: 11685_070316
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The samples were frozen in liquid nitrogen (-80° C) and extracted using a RNeasy Micro kit (Qiagen).
The cDNA library was generated according to the SMART-Seq2 protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
6418
|
| Data processing |
Sequencing data were demultiplexed using bcl2fastq2 v.2.2 and aligned to mouse reference genome (mm10).
Alignment and quantification with STAR v.2.5.1 using the default parameters
Normalization using DeSeq2
Genome_build: mm10
Supplementary_files_format_and_content: normalized txt files
|
| |
|
| Submission date |
Oct 07, 2020 |
| Last update date |
May 30, 2023 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE159187 |
Transcriptomic analysis of myocardium-specific Creld1 knockout mice at E13.5 and P3 |
|
| Relations |
| BioSample |
SAMN16391641 |
| SRA |
SRX9258690 |
