|
Status |
Public on May 30, 2023 |
Title |
KO2_vent |
Sample type |
SRA |
|
|
Source name |
KO_vent
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6N tissue: ventricles age: P3 genotype: MyHC-Creld1 KO pupid: 18318 #8 prepdate: 5/29/2018 litter: 18318_290518
|
Extracted molecule |
total RNA |
Extraction protocol |
The samples were frozen in liquid nitrogen (-80° C) and extracted using a RNeasy Micro kit (Qiagen). The cDNA library was generated according to the SMART-Seq2 protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
6434
|
Data processing |
Sequencing data were demultiplexed using bcl2fastq2 v.2.2 and aligned to mouse reference genome (mm10). Alignment and quantification with STAR v.2.5.1 using the default parameters Normalization using DeSeq2 Genome_build: mm10 Supplementary_files_format_and_content: normalized txt files
|
|
|
Submission date |
Oct 07, 2020 |
Last update date |
May 30, 2023 |
Contact name |
Joachim Schultze |
E-mail(s) |
j.schultze@uni-bonn.de
|
Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
Department |
Genomics and Immunoregulation
|
Street address |
Carl-Troll-Strasse 31
|
City |
Bonn |
State/province |
NRW |
ZIP/Postal code |
53115 |
Country |
Germany |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE159187 |
Transcriptomic analysis of myocardium-specific Creld1 knockout mice at E13.5 and P3 |
|
Relations |
BioSample |
SAMN16391625 |
SRA |
SRX9258706 |