|
| Status |
Public on Oct 06, 2023 |
| Title |
CD138+cells from normal_Replicate 2 [miRNA-Seq] |
| Sample type |
SRA |
| |
|
| Source name |
Bone marrow CD138+ cells
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Normal
cell type: CD138+ cells
gender: Male
age: 59
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bone marrow CD138+ cells were obtained from multiple myeloma patients and normal individuals by magnetic bead separation. The cells were treated with TRIzol to extract total RNA according to manufactures' instrctions.
miRNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
FastQC 0.11.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence (Cutadapt 1.14), and filtered for low-complexity or low-quality sequence, then mapped to GRCh38 whole genome using bowtie software.
Counts per million reads (CPM) were calculated using miRDeep2 0.0.8 software.
Genome_build: GRCh38
Supplementary_files_format_and_content: tab-delimited text files include CPM values for each Sample
|
| |
|
| Submission date |
Oct 08, 2020 |
| Last update date |
Oct 06, 2023 |
| Contact name |
Han Yan |
| E-mail(s) |
picachu122@126.com
|
| Organization name |
Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
|
| Street address |
Jiefang Avenue
|
| City |
Wuhan |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE159231 |
miRNA expression profiles of multiple myeloma |
| GSE159232 |
Multiple myeloma patients and control |
|
| Relations |
| BioSample |
SAMN16397406 |
| SRA |
SRX9264632 |
