|
| Status |
Public on Apr 15, 2022 |
| Title |
Non tumoral adjacent 3 |
| Sample type |
RNA |
| |
|
| Source name |
Non tumoral adjacent liver tissue3
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: Male
age: 55 weeks
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed with SV total RNA isolation system from Promega.
|
| Label |
biotin
|
| Label protocol |
250 ng of total RNA is reverse transcribed following the Genechip Whole transcript (WTplus) Sense Target labelling assay kit (Affymetrix). Briefly, the resulting double strand cDNA is used for in vitro transcription with T7 RNA pol. This cRNA is reverse transcribed in single stranded sens DNA which is fragmented and biotin labelled
|
| |
|
| Hybridization protocol |
biotynilated single stranded sense cDNA is then hybridized to GeneChip® MouseGene2.0ST (Affymetrix) at 45°C for 17 hours
|
| Scan protocol |
After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix) and scanned using the GCS3000 7G
|
| Description |
Non tumoral adjacent liver tissue from 55 weeks old C57BL/6J mice overexpressing ChREBP
|
| Data processing |
Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. Brain array CDF file V21 was used for annotation.
|
| |
|
| Submission date |
Oct 19, 2020 |
| Last update date |
Apr 15, 2022 |
| Contact name |
Franck Letourneur |
| E-mail(s) |
franck.letourneur@inserm.fr
|
| Organization name |
INSERM
|
| Department |
U1016
|
| Lab |
genomic
|
| Street address |
22 rue mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL23092 |
| Series (1) |
| GSE159518 |
Expression data from ChREBP overexpressing HCC tumors compared to non-tumoral adjacent liver tissue |
|
