|
| Status |
Public on Dec 11, 2009 |
| Title |
KLF2-1 [kinome] |
| Sample type |
protein |
| |
|
| Source name |
umbilical vein endothelial cells transduced with KLF2-expressing lentivirus
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: umbilical vein endothelial cell
protocol: KLF2-transduced
|
| Treatment protocol |
Cells were transduced by lentiviral vectors expressing no protein (mock) or Krueppel-like factor 2, and total cell protein was isolated at 72 h after transduction
|
| Growth protocol |
Human umbilical vein endothelial cells were grown in fibronectin coated tissue culture, using standard medium M199+20% FBS + ECGS
|
| Extracted molecule |
protein |
| Extraction protocol |
Protein was isolated according to the KINEXUS protocol (available on KINEXUS website)
|
| Label |
proprietary KINEX
|
| Label protocol |
Kinexus service
|
| |
|
| Hybridization protocol |
Kinexus service
|
| Scan protocol |
Kinexus service
|
| Description |
total cell protein lysate assayed for protein kinases
|
| Data processing |
Interarray quantile normalization performed by Kinexus service
|
| |
|
| Submission date |
Dec 11, 2009 |
| Last update date |
Dec 11, 2009 |
| Contact name |
Anton J Horrevoets |
| URL |
http://www.vumc.nl/afdelingen/moleculairecelbiologie
|
| Organization name |
VU University Medical Center
|
| Department |
Molecular Cell Biology
|
| Lab |
B244
|
| Street address |
van der Boechorststraat 7
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 BT |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL9787 |
| Series (1) |
| GSE19412 |
KLF2-induced actin shear fibers control both alignment to flow and JNK signaling in vascular endothelium |
|
