|
| Status |
Public on Apr 15, 2022 |
| Title |
Sample 8: IM-0223 EV |
| Sample type |
protein |
| |
|
| Source name |
Melanoma cells (cultured(
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: IM-0223
passages after establishing stable clones: 4
transfected vector: Control empty vector
|
| Growth protocol |
Cell lines were grown until 80% confluency and dettached using cell stripper non-enzimatic solution. Cells were washed with PBS and pelleted. Protein extraction were as described in extract protocol.
|
| Extracted molecule |
protein |
| Extraction protocol |
Protein extractions, quantification, and downstream RPPA analysis were performed by CCSG-supported RPPA Core Facility at the University of Texas MD Anderson Cancer Center.
|
| Label |
n/a
|
| Label protocol |
https://www.mdanderson.org/research/research-resources/core-facilities/functional-proteomics-rppa-core.html
|
| |
|
| Hybridization protocol |
https://www.mdanderson.org/research/research-resources/core-facilities/functional-proteomics-rppa-core.html
|
| Scan protocol |
https://www.mdanderson.org/research/research-resources/core-facilities/functional-proteomics-rppa-core.html
|
| Description |
control
|
| Data processing |
RPPA slides were assayed with 306 antibodies, analyzed by Array-Pro Analyzer 6.3 then by SuperCurve_1.5.0 via SuperCurveGUI_2.1.1
RPPA Core Facility at the University of Texas MD Anderson Cancer Center
Ratio was calculated as follow: (expression of gene X in OV- expression of gene X in contorl EV)/expression of gene X in control EV
Fold Change worksheet reports test/control (i.e., EV/OV) ratios.
|
| |
|
| Submission date |
Oct 20, 2020 |
| Last update date |
Apr 15, 2022 |
| Contact name |
Dave S.B. Hoon |
| E-mail(s) |
hoond@jwci.org
|
| Organization name |
Saint John's Cancer Institute
|
| Department |
Translational Molecular Medicine
|
| Street address |
2200 Santa Monica Boulevard
|
| City |
Santa Monica |
| State/province |
CA |
| ZIP/Postal code |
90403 |
| Country |
USA |
| |
|
| Platform ID |
GPL29258 |
| Series (1) |
| GSE159692 |
Interleukin enhancer-binding factor 2 promotes cell proliferation and DNA damage response in metastatic melanoma |
|
