|
| Status |
Public on Mar 03, 2021 |
| Title |
aorta_Veh_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
aorta, Vehicle, replicate 1
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
tissue: aorta
gender: male
age: 8-10 weeks old
|
| Treatment protocol |
Under ketamine/xylazine anesthesia (75 and 6 mg/kg), Ang Ⅱ- or PBS-loaded osmotic pumps were implanted in Male C57BL/6J mice (8-10 weeks old). Then the mice received berberine administration (100 mg/kg/day) or vehicle for 2 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After mice were sacrificed, aortae were removed, cleaned of adhering tissue in sterilized PBS, and then stored in liquid nitrogen for total RNA extraction. Total RNA from each sample was extracted using Trizol reagent (Invitrogen Life Technologies, Carlsbad, CA, USA). RNA quantity and quality were measured by NanoDrop ND-1000 to make sure the high purity of the isolated RNA.
|
| Label |
Cy3
|
| Label protocol |
The mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3’ bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen). The concentration and specific activity of the labeled cRNAs (pmol Cy3/μg cRNA) were measured by NanoDrop ND-1000.
|
| |
|
| Hybridization protocol |
1 μg of each labeled cRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA.
|
| Scan protocol |
50 μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide. The slides were incubated for 17 hours at 65°C in an Agilent Hybridization Oven. The hybridized arrays were washed, fixed and scanned with using the Agilent DNA Microarray Scanner (part number G2505C).
|
| Description |
Gene expression in aortae of Vehicle mice
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze the acquired array images. Quantile normalization and subsequent data processing were performed with the Gene Spring GX v12.1 software package (Agilent Technologies).
|
| |
|
| Submission date |
Oct 21, 2020 |
| Last update date |
Mar 03, 2021 |
| Contact name |
Na Tan |
| E-mail(s) |
tanna963@bjmu.edu.cn
|
| Phone |
0086 19800356860
|
| Organization name |
Peking University Health Science Center
|
| Street address |
No. 38 Xue Yuan Road
|
| City |
BeiJing |
| ZIP/Postal code |
100191 |
| Country |
China |
| |
|
| Platform ID |
GPL25015 |
| Series (2) |
| GSE159725 |
Berberine ameliorates vascular dysfunction by a global modulation of mRNA and lncRNA expression profiles in hypertensive mouse aortae |
| GSE165561 |
DPP-4 inhibitor MK-626 improves vascular endothelial function by modulating lncRNA and mRNA expression profiles in Ang II-induced hypertensive mouse aortae. |
|
