|
| Status |
Public on Oct 18, 2010 |
| Title |
Total RNA with small fraction Reference 2 251911910767_200906171315_S01_miRNA-v1_10_Apr08_1_4 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA with small fraction
|
| Organism |
Mus musculus |
| Characteristics |
sample type: Reference 2
source: pool of equal parts RNA from heart, lung and liver
internal id: Microarray Barcode 251911910767
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing the small fraction was isolated from each sample using the miRVana miRNA Isolation Kit (Ambion), Streetsville, ON, Canada). Quality was confirmed using the Agilent 2100 Bioanalyzer and RNA Nano 6000 chips.
|
| Label |
Cy3
|
| Label protocol |
Total RNA samples were labelled using the Agilent miRNA Complete Labelling and Hybridisation Kit (Agilent Tech, Mississauga, ON, Canada).
|
| |
|
| Hybridization protocol |
Hybridisations were performed in Agilent SureHyb chambers for 20 hours at 55C and washed according to manufacturer's instructions.
|
| Scan protocol |
Arrays were scanned at a resolution of 5 mm using an Agilent G2505B scanner.
|
| Description |
Reference RNA was developed from FirstChoice® mouse Total RNA including the small fraction (Catalogue # AM7800-AM7828, Ambion, Streetsville, ON). Two references were made from the various stocks. Reference # 1 was a pool of equal amounts of total RNA from mouse testicle, ovary and 10-12 day embryo. Reference #2 was a pool of equal parts total RNA from mouse liver, heart and lung.
|
| Data processing |
Cyclic Lowess
|
| |
|
| Submission date |
Dec 11, 2009 |
| Last update date |
Oct 18, 2010 |
| Contact name |
Andrew Williams |
| Organization name |
Health Canada
|
| Street address |
50 Columbine
|
| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A OK9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL7732 |
| Series (2) |
| GSE18841 |
Adult mouse liver following benzo(a)pyrene (BaP) treatment: miRNA and mRNa profiling |
| GSE19438 |
Reference: MicroRNA expression profiling in adult mouse liver following BaP treatment [Agilent miRNA array] |
|
