All human MSCs used for experiment were cultured in the MesenCultTM medium in the presence of 5% CO2, or in Dulbecco's modified Eagle's medium DMEM supplemented with 10% fetal bovine serum . For BM-MSCs isolation, the mononuclear fraction of bone marrow was isolated by Histopaque-1077 gradient (1077-1; Sigma, USA), and plated in culture medium. Non-adherent cells were abandoned on next day Total RNA were extracted by RNeasy mini kit (Cat. 74106; Qiagen, Hilden, Germany), and 100 ng to 1 ug of total RNA were subjected into reverse transcription using the SuperScriptTM III Reverse transcriptase kit (Invitrogen) as directed by the manufacturer.
Label
Biotin
Label protocol
standard Affymetrix procedures
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
BM-MSC from patient
Data processing
RMA through R based affy module
Submission date
Dec 14, 2009
Last update date
Oct 02, 2010
Contact name
Wei-Lun Hwang
Organization name
National Yang Ming Chiao Tung University
Department
Department of Biotechnology and Laboratory Science in Medicine
