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Sample GSM48557 Query DataSets for GSM48557
Status Public on Apr 21, 2005
Title R-3-C2-8
Sample type RNA
 
Channel 1
Source name cy3 Liver5
Organism Mus musculus
Extracted molecule total RNA
 
Channel 2
Source name cy5 Liver3
Organism Mus musculus
Extracted molecule total RNA
 
 
Description Each laboratory was provided with aliquots of two different RNA samples that were prepared in one of the Consortium laboratories - a sample prepared from mouse livers (Liver RNA), and a sample prepared from equal amounts of RNA isolated from five mouse tissues (liver, kidney, lung, brain and spleen: Pooled RNA).
 
Submission date Apr 19, 2005
Last update date May 27, 2005
Contact name Kenneth Lyon Phillips
E-mail(s) kphillips@icoria.com
Phone 919 425 3075
Organization name Paradigm Array Labs
Street address
City Research Triangle Park
State/province NC
ZIP/Postal code 27709
Country USA
 
Platform ID GPL922
Series (1)
GSE2458 Cross-platform comparability of microarray data

Data table header descriptions
ID_REF
PositionX Found coordinates of the feature centroid in pixels
PositionY Found coordinates of the feature centroid in pixels
VALUE (base 10) log(REDsignal/GREENsignal) per feature (processed signals used)
LogRatioError Error of the log ratio calculated according to the error model chosen
PValueLogRatio Significance level of the Log Ratio computed for a feature
gSurrogateUsed The green surrogate value used
rSurrogateUsed The red surrogate value used
gIsFound A boolean used to flag found (strong) features. The flag is applied independently in each channel
rIsFound A boolean used to flag found (strong) features. The flag is applied independently in each channel
gProcessedSignal Dye-normalized signal after surrogate algorithm, per channel, used for computation of log ratio
rProcessedSignal Dye-normalized signal after surrogate algorithm, per channel, used for computation of log ratio
gProcessedSigError Standard error of propagated feature signal, per channel
rProcessedSigError Standard error of propagated feature signal, per channel
gNumPixOLHi Number of outlier pixels per feature with intensity > upper threshold set via the pixel outlier rejection method
rNumPixOLHi Number of outlier pixels per feature with intensity > upper threshold set via the pixel outlier rejection method
gNumPixOLLo Number of outlier pixels per feature with intensity < lower threshold set via the pixel outlier rejection method
rNumPixOLLo Number of outlier pixels per feature with intensity < lower threshold set via the pixel outlier rejection method
gNumPix Total number of pixels used to compute feature statistics; ie. total number of inlier pixels/per spot; same in both channels
rNumPix Total number of pixels used to compute feature statistics; ie. total number of inlier pixels/per spot; same in both channels
gMeanSignal Raw mean signal of feature in green channel (inlier pixels)
rMeanSignal Raw mean signal of feature in red channel (inlier pixels)
gMedianSignal Median local background signal (local to corresponding feature) computed per channel (inlier pixels)
rMedianSignal Median local background signal (local to corresponding feature) computed per channel (inlier pixels)
gPixSDev Standard deviation of all inlier pixels per feature; this is computed independently in each channel
rPixSDev Standard deviation of all inlier pixels per feature; this is computed independently in each channel
gBGNumPix Total number of pixels used to compute local BG statistics per spot; ie. total number of BG inlier pixels; same in both channels
rBGNumPix Total number of pixels used to compute local BG statistics per spot; ie. total number of BG inlier pixels; same in both channels
gBGMeanSignal Mean local background signal (local to corresponding feature) computed per channel (inlier pixels)
rBGMeanSignal Mean local background signal (local to corresponding feature) computed per channel (inlier pixels)
gBGMedianSignal Median local background signal (local to corresponding feature) computed per channel (inlier pixels)
rBGMedianSignal Median local background signal (local to corresponding feature) computed per channel (inlier pixels)
gBGPixSDev Standard deviation of all inlier pixels per local BG of each feature, computed independently in each channel
rBGPixSDev Standard deviation of all inlier pixels per local BG of each feature, computed independently in each channel
gNumSatPix Total number of saturated pixels per feature, computed per channel
rNumSatPix Total number of saturated pixels per feature, computed per channel
gIsSaturated Boolean flag indicating if a feature is saturated or not. A feature is saturated IF 50% of the pixels in a feature are above the saturation threshold
rIsSaturated Boolean flag indicating if a feature is saturated or not. A feature is saturated IF 50% of the pixels in a feature are above the saturation threshold
PixCorrelation Ratio of estimated feature covariance in RedGreen space to product of feature Standard Deviation in Red Green space
BGPixCorrelation The same concept as above but in case of background
gIsFeatNonUnifOL Boolean flag indicating if a feature is a NonUniformity Outlier or not. A feature is non-uniform if the pixel noise of feature exceeds a threshold established for a "uniform" feature
rIsFeatNonUnifOL Boolean flag indicating if a feature is a NonUniformity Outlier or not. A feature is non-uniform if the pixel noise of feature exceeds a threshold established for a "uniform" feature
gIsBGNonUnifOL The same concept as above but in case of background
rIsBGNonUnifOL The same concept as above but in case of background
gIsFeatPopnOL Boolean flag indicating if a feature is a Population Outlier or not. Probes with replicate features on a microarray are examined using population statistics
rIsFeatPopnOL Boolean flag indicating if a feature is a Population Outlier or not. Probes with replicate features on a microarray are examined using population statistics
gIsBGPopnOL The same concept as above but in case of background
rIsBGPopnOL The same concept as above but in case of background
IsManualFlag Boolean is manual flag
gBGSubSignal Background-subtracted signal
rBGSubSignal Background-subtracted signal
gBGSubSigError Propagated standard error as computed on net g(r) background subtracted signal
rBGSubSigError Propagated standard error as computed on net g(r) background subtracted signal
BGSubSigCorrelation Ratio of estimated background-subtracted feature signal co-variance in RG space to product of background-subtracted feature Standard Deviation in RG space
gIsPosAndSignif Boolean flag, established via a 2-sided t-test, indicates if the mean signal of a feature is greater than the corresponding background (selected by user) and if this difference is significant
rIsPosAndSignif Boolean flag, established via a 2-sided t-test, indicates if the mean signal of a feature is greater than the corresponding background (selected by user) and if this difference is significant
gPValFeatEqBG P-value from t-test of significance between g(r) Mean signal and g(r) background (selected by user)
rPValFeatEqBG P-value from t-test of significance between g(r) Mean signal and g(r) background (selected by user)
gNumBGUsed Background used to subtract from the MeanSignal; variable also used in t-test
rNumBGUsed Background used to subtract from the MeanSignal; variable also used in t-test
gIsWellAboveBG Boolean flag indicating if a feature is WellAbove Background or not
rIsWellAboveBG Boolean flag indicating if a feature is WellAbove Background or not
IsUsedBGAdjust Boolean used to flag features used for computation of global BG offset
gBGUsed Background used to subtract fromthe MeanSignal; variable also used in t-test
rBGUsed Background used to subtract fromthe MeanSignal; variable also used in t-test
gBGSDUsed Standard deviation of background used in g(r) channel; variable also used in t-test and surrogate algorithms
rBGSDUsed Standard deviation of background used in g(r) channel; variable also used in t-test and surrogate algorithms
IsNormalization Boolean flag which indicates if a feature is used to measure dye bias
gDyeNormSignal The dye-normalized signal in the indicated channel
rDyeNormSignal The dye-normalized signal in the indicated channel
gDyeNormError The standard error associated with the dye-normalized signal
rDyeNormError The standard error associated with the dye-normalized signal
DyeNormCorrelation Dye-normalized red and green pixel correlation
ErrorModel Indicates the error model that you chose for feature extraction or that the software uses if you have chosen the "most conservative" option

Data table
ID_REF PositionX PositionY VALUE LogRatioError PValueLogRatio gSurrogateUsed rSurrogateUsed gIsFound rIsFound gProcessedSignal rProcessedSignal gProcessedSigError rProcessedSigError gNumPixOLHi rNumPixOLHi gNumPixOLLo rNumPixOLLo gNumPix rNumPix gMeanSignal rMeanSignal gMedianSignal rMedianSignal gPixSDev rPixSDev gBGNumPix rBGNumPix gBGMeanSignal rBGMeanSignal gBGMedianSignal rBGMedianSignal gBGPixSDev rBGPixSDev gNumSatPix rNumSatPix gIsSaturated rIsSaturated PixCorrelation BGPixCorrelation gIsFeatNonUnifOL rIsFeatNonUnifOL gIsBGNonUnifOL rIsBGNonUnifOL gIsFeatPopnOL rIsFeatPopnOL gIsBGPopnOL rIsBGPopnOL IsManualFlag gBGSubSignal rBGSubSignal gBGSubSigError rBGSubSigError BGSubSigCorrelation gIsPosAndSignif rIsPosAndSignif gPValFeatEqBG rPValFeatEqBG gNumBGUsed rNumBGUsed gIsWellAboveBG rIsWellAboveBG IsUsedBGAdjust gBGUsed rBGUsed gBGSDUsed rBGSDUsed IsNormalization gDyeNormSignal rDyeNormSignal gDyeNormError rDyeNormError DyeNormCorrelation ErrorModel
22575 78.5318 54.5333 -2 0.503028 7.01072e-005 0 0 1 1 100512 604.327 990.05 28.487 0 2 0 0 58 58 17588.6 213.759 17315.5 214 1286.05 6.65519 263 263 400.909 192.521 401 192 3.57462 4.87932 0 0 0 0 0.157279 -5.69944e-005 0 0 0 0 0 0 0 0 0 17143.6 19.6064 168.867 0.924214 0.148711 1 1 4.20132e-066 1.78794e-032 1 1 1 1 0 444.928 194.152 3.57462 4.87932 0 100512 604.327 990.05 28.487 0.148711 1
22574 99.6195 53.354 0 0.109903 1 35.6587 88.4071 1 1 35.6587 88.4071 7.6645 12.5319 1 1 0 0 57 57 436.175 197.228 437 198 5.26824 5.00363 278 278 401.014 192.709 401 192 3.38074 5.16004 0 0 0 0 0.0424876 1.23175e-007 0 0 0 0 1 0 0 0 0 -8.85863 2.8881 0.726658 0.731444 0.0369681 0 1 1.5606e-018 0.000165068 1 1 0 0 0 445.034 194.34 3.38074 5.16004 0 -93.4373 49.4819 7.6645 12.5319 0.0369681 0
22573 120.714 54.2942 -0.0940484 0.134147 0.48325 0 0 1 1 4367.19 3516.85 45.3889 66.1261 1 2 1 0 58 58 1199.69 307.207 1203 306 59.6411 15.9481 279 279 401.839 192.832 402 193 3.87379 5.50705 0 0 0 0 0.190214 0.00027282 0 0 0 0 0 0 0 0 0 753.831 112.744 7.83469 2.11989 0.187819 1 1 7.16173e-065 4.28953e-052 1 1 1 1 0 445.858 194.463 3.87379 5.50705 1 4367.19 3516.85 45.3889 66.1261 0.187819 1
22572 141.908 54.1157 -0.0141724 0.128098 0.911904 0 0 1 1 1122.48 1086.44 18.1214 43.7561 0 1 1 0 60 60 651.517 228.133 648 229 25.6373 9.89002 272 272 401.824 193.691 401 193 4.38192 5.6176 0 0 0 0 0.0458478 0.000323054 0 0 0 0 0 0 0 0 0 205.673 32.8108 3.32041 1.32145 0.0441632 1 1 6.35337e-056 1.06058e-035 1 1 1 1 0 445.843 195.323 4.38192 5.6176 1 1122.48 1086.44 18.1214 43.7561 0.0441632 1
22571 163.545 54.8866 0.285847 0.139796 0.0408795 0 0 1 1 114.505 221.142 9.763 33.1825 0 4 0 0 55 55 469.473 200.236 467 201 14.8085 6.25082 277 277 401.866 192.664 402 193 3.98187 4.82962 0 0 0 0 -0.0438421 -0.000207056 0 0 0 0 0 0 0 0 0 23.5866 5.94077 2.01106 0.891415 -0.0411677 1 1 1.16882e-016 5.88177e-009 1 1 1 0 0 445.886 194.296 3.98187 4.82962 0 114.505 221.142 9.763 33.1825 -0.0411677 1
22570 184.822 54.3129 0.0452853 0.125209 0.717592 0 0 1 1 614.268 681.778 15.0817 32.8221 0 0 0 0 61 61 562.148 214.852 561 215 22.321 7.08481 279 279 401.391 193.369 401 193 3.63061 5.02547 0 0 0 0 0.172666 -0.000210747 0 0 0 0 0 0 0 0 0 116.737 19.8519 2.86617 0.95571 0.16341 1 1 8.77088e-046 1.53575e-032 1 1 1 1 0 445.41 195.001 3.63061 5.02547 1 614.268 681.778 15.0817 32.8221 0.16341 1
22569 205.629 55.7718 -2 0.503143 7.03762e-005 0 0 1 1 96106.5 599.684 935.361 29.7191 1 2 0 0 57 57 16844.9 214.263 16697 214 1204.96 6.81785 283 283 402.163 193.184 402 193 4.16781 5.66576 0 0 0 0 0.103414 -0.000176412 0 0 0 0 0 0 0 0 0 16398.7 19.4481 159.602 0.963807 0.0968942 1 1 1.78813e-065 1.92537e-031 1 1 1 1 0 446.182 194.815 4.16781 5.66576 0 96106.5 599.684 935.361 29.7191 0.0968942 1
22568 226.954 54.3945 0 0.0915678 1 0 180.023 1 1 44.7026 180.023 9.18399 23.8154 2 3 0 0 55 55 453.527 195.836 452 196 11.8881 4.95073 279 279 401.638 193.208 402 193 3.51893 5.65874 0 0 0 0 0.317398 0.000195534 0 0 0 0 0 0 0 0 0 7.8696 0.997144 1.61678 0.748601 0.280634 1 0 9.6458e-006 0.186447 1 1 0 0 0 445.658 194.839 3.51893 5.65874 0 44.7026 31.7224 9.18399 23.8154 0.280634 0
22567 248 54.8435 0 0.12147 1 45.0457 94.7204 1 1 45.0457 94.7204 10.2032 14.6262 1 2 0 0 60 60 445.133 196.983 445 196.5 7.22159 6.08553 274 274 402.391 193.464 402 193 4.2707 5.52853 0 0 0 0 -0.0497001 0.000555682 0 0 0 0 0 0 0 0 0 -1.27686 1.8885 0.967345 0.853684 -0.0440238 0 0 0.191255 0.0297473 1 1 0 0 0 446.41 195.095 4.2707 5.52853 0 -13.4678 32.3556 10.2032 14.6262 -0.0440238 0
22566 269.314 54.9317 0.183407 0.118669 0.122217 0 0 1 1 1039.6 1585.9 36.6111 53.8863 9 8 0 0 51 51 636.098 242.392 625 242 47.3575 11.4927 258 258 403.566 192.364 404 192 5.02266 5.43076 0 0 0 0 0.109947 0.00037587 0 0 0 0 0 0 0 0 0 188.512 48.3965 6.63874 1.64444 0.107483 1 1 1.34454e-032 3.92307e-035 1 1 1 1 0 447.586 193.996 5.02266 5.43076 0 1039.6 1585.9 36.6111 53.8863 0.107483 1
22565 289.874 54.7149 0.242359 0.130668 0.0636282 0 0 1 1 160.314 280.111 7.92531 38.3584 0 0 0 0 62 62 479.226 201.726 477.5 202 12.5964 7.71664 265 265 402.396 192.521 402 192 4.35864 5.52616 0 0 0 0 -0.00137643 0.000566925 0 0 0 0 0 0 0 0 0 32.8099 7.57372 1.622 1.03714 -0.00125214 1 1 1.29351e-029 2.31528e-010 1 1 1 0 0 446.416 194.152 4.35864 5.52616 0 160.314 280.111 7.92531 38.3584 -0.00125214 1
22564 311.57 54.4298 0 0.075147 1 0 210.234 1 1 81.3718 210.234 8.10048 31.8336 2 1 0 0 56 56 462.768 198.946 462 198.5 12.0785 6.03451 284 284 402.394 193.07 402 193 3.58335 5.78856 0 0 0 0 0.105343 0.000648495 0 0 0 0 0 0 0 0 0 16.3538 4.24467 1.62801 0.876502 0.0961203 1 1 3.23429e-014 6.55694e-006 1 1 1 0 0 446.414 194.702 3.58335 5.78856 0 81.3718 154.162 8.10048 31.8336 0.0961203 0
22563 332.514 55.5096 0.0938695 0.137242 0.493994 0 0 1 1 171.265 212.588 8.43378 30.5568 0 1 0 0 59 59 481.288 199.915 484 201 13.2275 5.79324 266 266 402.023 192.568 402 192 3.53306 5.31615 0 0 0 0 -0.107673 9.93941e-005 0 0 0 0 0 0 0 0 0 35.2459 5.71625 1.73565 0.821637 -0.0980601 1 1 1.56868e-028 8.12961e-010 1 1 1 0 0 446.042 194.199 3.53306 5.31615 0 171.265 212.588 8.43378 30.5568 -0.0980601 1
22562 353.673 55.3434 -2 0.502955 6.99366e-005 0 0 1 1 95150.9 558.327 866.934 31.0419 2 0 0 0 59 59 16691.8 212.932 16483 213 1136.93 7.27979 273 273 402.187 193.205 402 193 3.89167 5.57849 0 0 0 0 0.242555 -6.29386e-005 0 0 0 0 0 0 0 0 0 16245.6 18.0957 148.016 1.00609 0.228489 1 1 5.66553e-069 1.28648e-028 1 1 1 1 0 446.206 194.836 3.89167 5.57849 0 95150.9 558.327 866.934 31.0419 0.228489 1
22561 374.684 55.8718 0 0.0736498 1 0 202.543 1 1 202.424 202.543 11.1436 33.7965 0 0 0 0 61 61 486.689 199.197 484 198 17.8097 6.63531 282 282 401.043 192.56 401 193 3.81022 5.45035 0 0 0 0 0.123793 8.67551e-006 0 0 0 0 0 0 0 0 0 41.6263 5.0051 2.29156 0.909451 0.115073 1 1 2.46779e-026 4.53248e-007 1 1 1 0 0 445.062 194.192 3.81022 5.45035 1 202.424 185.997 11.1436 33.7965 0.115073 0
22560 396.252 55.8571 0 0.0776399 1 0 177.335 1 1 77.1777 177.335 8.6945 23.7087 3 4 0 1 55 55 461.345 198.836 460 199 12.7791 4.3066 262 262 401.863 193.019 402 193 4.14075 4.89777 0 0 0 0 -0.0527923 0.000504528 0 0 0 0 0 0 0 0 0 15.4632 4.18594 1.74201 0.654807 -0.0462759 1 1 2.68796e-012 8.11537e-009 1 1 1 0 0 445.882 194.65 4.14075 4.89777 0 77.1777 151.561 8.6945 23.7087 -0.0462759 0
22559 417.102 55.7203 0 0.0850612 1 0 171.235 1 1 40.1021 171.235 5.80336 26.0323 0 2 0 2 58 58 453.103 198.086 453 198 8.30491 5.22908 267 267 401.382 192.798 401 193 3.76421 4.9338 0 0 0 0 0.144417 0.000494786 0 0 0 0 0 0 0 0 0 7.70175 3.65712 1.11456 0.750071 0.129386 1 1 3.03716e-009 5.35978e-006 1 1 0 0 0 445.402 194.429 3.76421 4.9338 0 40.1021 126.926 5.80336 26.0323 0.129386 0
22558 438.475 55.9582 0.330401 0.135585 0.0148159 0 0 1 1 122.519 262.184 7.14738 33.3072 4 0 1 1 54 54 470.204 201.944 469 202 10.6919 6.0918 278 278 400.921 193.277 401 194 3.91159 5.57381 0 0 0 0 -0.0800654 1.3755e-005 0 0 0 0 0 0 0 0 0 25.2632 7.03613 1.47377 0.893855 -0.0733075 1 1 6.76713e-024 2.82553e-011 1 1 1 0 0 444.941 194.908 3.91159 5.57381 0 122.519 262.184 7.14738 33.3072 -0.0733075 1
22557 459.712 56.36 0.168523 0.126328 0.1822 0 0 1 1 231.663 341.492 9.80605 33.5984 1 0 0 0 59 59 491.017 203.966 489 204 15.0339 6.71582 284 284 400.504 192.919 400 193 3.46767 5.16003 0 0 0 0 -0.0221938 0.000291062 0 0 0 0 0 0 0 0 0 46.4937 9.41575 1.96803 0.92639 -0.0208216 1 1 7.3965e-032 1.29351e-015 1 1 1 0 0 444.523 194.55 3.46767 5.16003 0 231.663 341.492 9.80605 33.5984 -0.0208216 1
22556 480.555 55.7311 0 0.0700954 1 0 183.412 1 1 69.2453 183.412 7.57997 29.1841 1 1 0 0 57 57 459 197.86 458 198 11.0873 5.75834 281 281 401.388 193.139 401 193 4.00924 5.17052 0 0 0 0 0.352978 -9.87896e-005 0 0 0 0 0 0 0 0 0 13.5924 3.08952 1.4879 0.82272 0.322971 1 1 6.80006e-013 0.000338649 1 1 1 0 0 445.408 194.77 4.00924 5.17052 1 69.2453 109.594 7.57997 29.1841 0.322971 0

Total number of rows: 21446

Table truncated, full table size 8278 Kbytes.




Supplementary data files not provided

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