|
| Status |
Public on Jan 01, 2010 |
| Title |
WI-38 cells, at 24 h after exposure to 0.5 uM BPDE, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
WI-38 cells exposed to 0.5 uM BPDE for 24 h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Normal human diploid WI-38 lung fibroblasts, CCL-75(TM), passage 7-12.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was prepared using the RNeasy Mini Kit from (Qiagen), treated with TURBO DNA-freeTM (Ambion) and RNA integrity analyzed using Agilent Bioanalyzer 2100.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix Protocols
|
| |
|
| Hybridization protocol |
Standard Affymetrix Protocols
|
| Scan protocol |
The microarrays were scanned using a GeneChip® Scanner 3000 (Affymetrix) and subsequent image analysis was performed using Affymetrix GCOS software using standard Affymetrix protocols.
|
| Description |
Gene expression data from WI-38 cells exposed to DMSO vehicle control
|
| Data processing |
The resulting .cel files were normalized with the robust multichip average (RMA) method.
|
| |
|
| Submission date |
Dec 16, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Kristian Dreij |
| E-mail(s) |
kristian.dreij@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Environmental Medicine
|
| Street address |
Nobels väg 13
|
| City |
Stockholm |
| ZIP/Postal code |
17177 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE19510 |
Transcriptional response of normal human lung WI-38 fibroblasts to benzo[a]pyrene diol epoxide: a dose-response study |
|
| Relations |
| Reanalyzed by |
GSE86362 |
| Reanalyzed by |
GSE119087 |
