|
Status |
Public on Aug 01, 2021 |
Title |
Mammary carcinoma, #12 |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Mammary carcinoma induced by radiation
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: Carcinoma gender: Female strain: F1 hybrid of Sprague-Dawley and Copenhagen rats
|
Treatment protocol |
Rats were left untreated or were subjected to whole-body 137Cs gamma-irradiation (4 Gy, 0.5 Gy/min) at 7 weeks of age.
|
Growth protocol |
Rats were housed in autoclaved cages and maintained in a room with a controlled temperature (23 ± 1°C) and humidity (50 ± 5%) under a regular 12-hours light, 12-hours dark cycle. They were fed a standard laboratory animal diet (CE-2; Clea Japan, Tokyo, Japan) and sterilized water ad libitum.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated by proteinase K digestion, followed by phenol/chloroform extraction.
|
Label |
Cy5
|
Label protocol |
Labeling reactions with cyanine 5-UTP and cyanine 3-UTP were performed according to the Agilent SureTag DNA labeling kit (Agilent Technologies, Santa Clara, CA, USA).
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|
|
Channel 2 |
Source name |
Normal ear
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: Ear skin gender: Female strain: F1 hybrid of Sprague-Dawley and Copenhagen rats
|
Treatment protocol |
Rats were left untreated or were subjected to whole-body 137Cs gamma-irradiation (4 Gy, 0.5 Gy/min) at 7 weeks of age.
|
Growth protocol |
Rats were housed in autoclaved cages and maintained in a room with a controlled temperature (23 ± 1°C) and humidity (50 ± 5%) under a regular 12-hours light, 12-hours dark cycle. They were fed a standard laboratory animal diet (CE-2; Clea Japan, Tokyo, Japan) and sterilized water ad libitum.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Genomic DNA was isolated by proteinase K digestion, followed by phenol/chloroform extraction.
|
Label |
Cy3
|
Label protocol |
Labeling reactions with cyanine 5-UTP and cyanine 3-UTP were performed according to the Agilent SureTag DNA labeling kit (Agilent Technologies, Santa Clara, CA, USA).
|
|
|
|
Hybridization protocol |
Slides were hybridised in 1X Agilent Blocking Agent/Hi-RPM Buffer for 40 hours at 65°C in a rotating hybridization oven, then washed with the Agilent Oligo aCGH Buffer 1 and Buffer 2 in an ozone-depleted hood.
|
Scan protocol |
Scanned on an Agilent microarray scanner.
|
Description |
12_J24
|
Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
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|
|
Submission date |
Oct 30, 2020 |
Last update date |
Aug 02, 2021 |
Contact name |
Kazuhiro DAINO |
E-mail(s) |
daino.kazuhiro@qst.go.jp
|
Organization name |
National Institutes for Quantum and Radiological Science and Technology
|
Street address |
4-9-1 Anagawa, Inage-ku
|
City |
Chiba |
ZIP/Postal code |
263-8555 |
Country |
Japan |
|
|
Platform ID |
GPL29326 |
Series (1) |
GSE160514 |
DNA copy number alterations in radiation-induced rat mammary carcinomas |
|