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Sample GSM4875140 Query DataSets for GSM4875140
Status Public on Sep 26, 2021
Title LLR001_sgNTC_iTreg
Sample type RNA
 
Source name sgNTC-transduced iTreg cells at steady state
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: sgNTC-transduced iTreg cells at steady state
Treatment protocol Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
Growth protocol iTreg in vitro differentiation, plate-bound condition
Extracted molecule total RNA
Extraction protocol Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
Label biotin
Label protocol Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
 
Hybridization protocol Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Clariom S mouse GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
Data processing Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
 
Submission date Oct 30, 2020
Last update date Sep 27, 2021
Contact name Hongbo Chi
E-mail(s) hongbo.chi@stjude.org
Organization name St Jude Children's Research Hospital
Department Immunology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL23038
Series (2)
GSE160550 CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity [steady state]
GSE160598 CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
TC0100000014.mm.2 8.550451
TC0100000018.mm.2 4.575539
TC0100000021.mm.2 7.22289
TC0100000022.mm.2 3.695335
TC0100000023.mm.2 3.941238
TC0100000027.mm.2 8.940717
TC0100000038.mm.2 8.317668
TC0100000039.mm.2 5.159762
TC0100000041.mm.2 5.076569
TC0100000044.mm.2 8.279058
TC0100000045.mm.2 3.950164
TC0100000048.mm.2 6.894054
TC0100000055.mm.2 4.548044
TC0100000056.mm.2 4.701155
TC0100000064.mm.2 4.44918
TC0100000071.mm.2 3.628595
TC0100000082.mm.2 4.497285
TC0100000092.mm.2 10.16467
TC0100000097.mm.2 6.432633
TC0100000101.mm.2 5.985026

Total number of rows: 28846

Table truncated, full table size 777 Kbytes.




Supplementary file Size Download File type/resource
GSM4875140_LLR001_1576174_ClariomSmouse.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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