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Sample GSM4875150 Query DataSets for GSM4875150
Status Public on Sep 26, 2021
Title LLR013_sgNTC_iTreg
Sample type RNA
 
Source name sgNTC-transduced iTreg cells after 20hrs' aCD3/aCD28 (0.25/5 ug) restimulation
Organism Mus musculus
Characteristics strain: C57BL/6
genotype/variation: sgNTC-transduced iTreg cells
treatment: 20hrs' aCD3/aCD28 (0.25/5 ug) restimulation
Treatment protocol Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
Growth protocol iTreg in vitro differentiation, plate-bound condition
Extracted molecule total RNA
Extraction protocol Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
Label biotin
Label protocol Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
 
Hybridization protocol Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Clariom S mouse GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
Data processing Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
 
Submission date Oct 30, 2020
Last update date Sep 27, 2021
Contact name Hongbo Chi
E-mail(s) hongbo.chi@stjude.org
Organization name St Jude Children's Research Hospital
Department Immunology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
 
Platform ID GPL23038
Series (2)
GSE160552 CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity [TCR stimulation]
GSE160598 CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity

Data table header descriptions
ID_REF
VALUE log2 RMA signal

Data table
ID_REF VALUE
TC0100000014.mm.2 8.338035
TC0100000018.mm.2 5.236025
TC0100000021.mm.2 6.284141
TC0100000022.mm.2 3.40526
TC0100000023.mm.2 3.525124
TC0100000027.mm.2 8.359447
TC0100000038.mm.2 9.748944
TC0100000039.mm.2 4.773479
TC0100000041.mm.2 5.40391
TC0100000044.mm.2 7.715464
TC0100000045.mm.2 4.273335
TC0100000048.mm.2 7.391551
TC0100000055.mm.2 4.789962
TC0100000056.mm.2 4.307902
TC0100000064.mm.2 4.299485
TC0100000071.mm.2 4.079029
TC0100000082.mm.2 4.003134
TC0100000092.mm.2 9.512191
TC0100000097.mm.2 6.0145
TC0100000101.mm.2 7.537565

Total number of rows: 28846

Table truncated, full table size 777 Kbytes.




Supplementary file Size Download File type/resource
GSM4875150_LLR013_1845232_ClariomSmouse.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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