|
| Status |
Public on Sep 26, 2021 |
| Title |
LLR014_sgNTC_iTreg |
| Sample type |
RNA |
| |
|
| Source name |
sgNTC-transduced iTreg cells after 20hrs' aCD3/aCD28 (0.25/5 ug) restimulation
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
genotype/variation: sgNTC-transduced iTreg cells
treatment: 20hrs' aCD3/aCD28 (0.25/5 ug) restimulation
|
| Treatment protocol |
Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
|
| Growth protocol |
iTreg in vitro differentiation, plate-bound condition
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Clariom S mouse GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Oct 30, 2020 |
| Last update date |
Sep 27, 2021 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE160552 |
CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity [TCR stimulation] |
| GSE160598 |
CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity |
|
