|
| Status |
Public on Nov 01, 2020 |
| Title |
SUDEP DBA/1 mice-1 |
| Sample type |
RNA |
| |
|
| Source name |
Brain tissue
|
| Organism |
Mus musculus |
| Characteristics |
gender: male
age: 5 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
mRNA was purified from total RNA after removal of rRNA (mRNA-ONLY™ Eukaryotic mRNA Isolation Kit, Epicentre). Then, each sample was amplified and transcribed into fluorescent cRNA along the entire length of the transcripts without 3’ bias utilizing a random priming method (Arraystar Flash RNA Labeling Kit, Arraystar). The labeled cRNAs were purified by RNeasy Mini Kit (Qiagen).
|
| |
|
| Hybridization protocol |
1 μg of each labeled cRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min, finally 25 μl 2 × GE Hybridization buffer was added to dilute the labeled cRNA. 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the LncRNA expression microarray slide.
|
| Scan protocol |
Scanned with using the Agilent DNA Microarray Scanner (part number G2505C).
|
| Description |
Biological replicate 1 of 4
|
| Data processing |
Agilent Feature Extraction software (version 11.0.1.1) was used to analyze acquired array images. Quantile normalization and subsequent data processing were performed with using the GeneSpring GX v12.1 software package (Agilent Technologies).
Differentially expressed LncRNAs and mRNAs with statistical significance between the two groups were identified through P-value/FDR.
|
| |
|
| Submission date |
Oct 31, 2020 |
| Last update date |
Nov 01, 2020 |
| Contact name |
Ling Liu |
| E-mail(s) |
neurologyliuling@126.com
|
| Phone |
18980601676
|
| Organization name |
West China Hospital, Sichuan University
|
| Department |
Department of neurology
|
| Street address |
Wai Nan Guo Xue Lane 37 #
|
| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610041 |
| Country |
China |
| |
|
| Platform ID |
GPL19286 |
| Series (1) |
| GSE160576 |
Dysregulated long noncoding RNAs in the brainstem of DBA/1 mouse SUDEP model |
|
