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| Status |
Public on Nov 03, 2022 |
| Title |
ISFC2 |
| Sample type |
SRA |
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| Source name |
spleen
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| Organism |
Mus musculus |
| Characteristics |
tissue: spleen
agent: Busulfan+Con-FMT
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| Treatment protocol |
Oral dosing
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from spleen
RNA libraries were prepared for sequencing using standard BGISEQ protocols
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
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| Data processing |
The instrument model is BGISEQ-500
The original sequencing data contained reads with low quality, joint contamination and high unknown base N content. These reads needed to be removed before data analysis to ensure the reliability of the results
After clean reads were obtained, clean reads were aligned to the reference genome sequence using HISAT
Clean reads were aligned to the reference gene sequence using Bowtie2 to obtain alignment results.
Genome_build: mm10
Supplementary_files_format_and_content: Matrix table with raw gene counts for every gene and every sample
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| Submission date |
Nov 06, 2020 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Yong Zhao |
| E-mail(s) |
yzhao818@hotmail.com
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| Phone |
86-18661613617
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| Organization name |
Qingdao Agricultural University
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| Department |
College of Life Sciences
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| Lab |
B604
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| Street address |
700 Changcheng Road
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| City |
Qingdao |
| State/province |
Shandong |
| ZIP/Postal code |
266109 |
| Country |
China |
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| Platform ID |
GPL23479 |
| Series (1) |
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| Relations |
| BioSample |
SAMN16686443 |
| SRA |
SRX9450309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4887030_ISFC2.txt.gz |
794.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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