|
| Status |
Public on May 18, 2022 |
| Title |
PKD/Mhm (Cy/+) + i.p. ABCB5+ [ipABCB5_30] |
| Sample type |
SRA |
| |
|
| Source name |
Kidney
|
| Organism |
Rattus norvegicus |
| Characteristics |
treatment: ipABCB5
strain: PKD/Mhm (Cy/+)
age: 8 months
|
| Treatment protocol |
Stem cells injection (i.p. or i.v.) once per month for 6 months
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Left kidney was removed and snap frozen in liquid nitrogen. RNA was isolated with Rneasy mini kit Qiagen
RNA libraries were prepared for sequencing using standard BGISeq-500 protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Description |
ipABCB5_30
|
| Data processing |
trimmed with trim_galore version 0.6.4. Parameters: --length 26 --phred33
Get raw counts with kallisto quant version 0.4.6.
TPM abundance estimates, generated with kallisto quant 0.4.6
Genome_build: rn6
Supplementary_files_format_and_content: TPM abundance estimates and raw counts generated with kallisto.
|
| |
|
| Submission date |
Nov 09, 2020 |
| Last update date |
May 18, 2022 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24782 |
| Series (2) |
| GSE161091 |
Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats [PKD_Dez19] |
| GSE161093 |
Potential therapeutic effects of long-term stem cells administration: impact on the gene profile and kidney function of PKD/Mhm (Cy/+) rats |
|
| Relations |
| BioSample |
SAMN16709326 |
| SRA |
SRX9461590 |
