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Sample GSM489273 Query DataSets for GSM489273
Status Public on Jun 01, 2011
Title Wild Type Replicate Four
Sample type RNA
 
Channel 1
Source name BALB WT Control
Organism Mus musculus
Characteristics genotype: Balbc/J Cftrtm1UNC +/+
gender: male
tissue type: Ileum
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy3
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
Channel 2
Source name Control RNA from Exiqon
Organism Mus musculus
Characteristics reference: Control RNA from Exiqon
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy5
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
 
Hybridization protocol The Hy3TM and Hy5TM labelled samples were mixed pairwise and hybridization was performed according to the miRCURYTM LNA array manual (Exiqon) using a Tecan HS4800 hybridization station
Scan protocol Microarray slides were scanned using the Agilent G2565BA Microarray Scanner System. Image Analysis used ImaGene 8.0 software.
Description raw data file Hy3: 1_Exiqon_13978197_S01_Cropped.txt
raw data file Hy5: 0_Exiqon_13978197_S01_Cropped.txt
Biological Replicate 4 of 7 for BALB WT mice
Data processing LOWESS normalized and background corrected (Normexp with offset value 10)
 
Submission date Dec 23, 2009
Last update date Jun 01, 2011
Contact name Christina Kathleen Haston
E-mail(s) christina.haston@mcgill.ca
Phone 514-398-3864 089714
Organization name McGill University
Department Medicine
Street address 3626 St. Urbain
City Montreal
State/province QC
ZIP/Postal code H2X 2P2
Country Canada
 
Platform ID GPL7723
Series (1)
GSE19621 MicroRNA profiling of cystic fibrosis intestinal disease in mice

Data table header descriptions
ID_REF
VALUE log2(Hy3/Hy5) ratios, representing sample/reference

Data table
ID_REF VALUE
14306 -0.271696439758
11078 0.534661296167
10986 0.101176281281
19582 0.277212838604
11208 0.305304435828
19599 0.208883772153
46621 0.199388002803
46793 -0.104693055403
42724 -0.051363609558
10998 0.177472237934
46730 0.334148868925
42471 -0.248718283567
10967 0.410720502616
5740 0.113098096527
13143 0.089843373369
10985 0.390886878983
27575 0.087930773303
46636 0.038725011524
10997 -0.037822936211
42682 0.326077861252

Total number of rows: 657

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM489273_0_Exiqon_13978197_S01_Cropped.txt.gz 775.9 Kb (ftp)(http) TXT
GSM489273_1_Exiqon_13978197_S01_Cropped.txt.gz 864.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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