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Sample GSM489275 Query DataSets for GSM489275
Status Public on Jun 01, 2011
Title Wild Type Replicate Six
Sample type RNA
 
Channel 1
Source name BALB WT Control
Organism Mus musculus
Characteristics genotype: Balbc/J Cftrtm1UNC +/+
gender: female
tissue type: Ileum
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy3
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
Channel 2
Source name Control RNA from Exiqon
Organism Mus musculus
Characteristics reference: Control RNA from Exiqon
Growth protocol All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
Extracted molecule total RNA
Extraction protocol Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
Label Hy5
Label protocol 1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
 
 
Hybridization protocol The Hy3TM and Hy5TM labelled samples were mixed pairwise and hybridization was performed according to the miRCURYTM LNA array manual (Exiqon) using a Tecan HS4800 hybridization station
Scan protocol Microarray slides were scanned using the Agilent G2565BA Microarray Scanner System. Image Analysis used ImaGene 8.0 software.
Description raw data file Hy3: 1_Exiqon_13978199_S01_Cropped.txt
raw data file Hy5: 0_Exiqon_13978199_S01_Cropped.txt
Biological Replicate 6 of 7 for BALB WT mice
Data processing LOWESS normalized and background corrected (Normexp with offset value 10)
 
Submission date Dec 23, 2009
Last update date Jun 01, 2011
Contact name Christina Kathleen Haston
E-mail(s) christina.haston@mcgill.ca
Phone 514-398-3864 089714
Organization name McGill University
Department Medicine
Street address 3626 St. Urbain
City Montreal
State/province QC
ZIP/Postal code H2X 2P2
Country Canada
 
Platform ID GPL7723
Series (1)
GSE19621 MicroRNA profiling of cystic fibrosis intestinal disease in mice

Data table header descriptions
ID_REF
VALUE log2(Hy3/Hy5) ratios, representing sample/reference

Data table
ID_REF VALUE
14306 -0.186290646722
11078 0.119021789661
10986 -0.270279300025
19582 0.141491576803
11208 0.280304626702
19599 0.240038677331
46621 0.157983216784
46793 0.177669412311
42724 -0.146480799325
10998 0.031174221626
46730 0.203859864399
42471 -0.165032198139
10967 0.126998629155
5740 -0.308430770794
13143 0.060184341112
10985 0.294918493146
27575 0.229539704578
46636 0.123157439235
10997 0.128366175537
42682 0.357053057081

Total number of rows: 657

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM489275_0_Exiqon_13978199_S01_Cropped.txt.gz 771.6 Kb (ftp)(http) TXT
GSM489275_1_Exiqon_13978199_S01_Cropped.txt.gz 864.1 Kb (ftp)(http) TXT
Processed data included within Sample table

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