|
Status |
Public on Jun 01, 2011 |
Title |
Wild Type Replicate Six |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
BALB WT Control
|
Organism |
Mus musculus |
Characteristics |
genotype: Balbc/J Cftrtm1UNC +/+ gender: female tissue type: Ileum
|
Growth protocol |
All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
|
Label |
Hy3
|
Label protocol |
1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
|
|
|
Channel 2 |
Source name |
Control RNA from Exiqon
|
Organism |
Mus musculus |
Characteristics |
reference: Control RNA from Exiqon
|
Growth protocol |
All mice were maintained on Colyte from 3 weeks to age of euthanasia, at 12 weeks
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA from mouse ileum was extracted using miRNeasy Mini kits from Qiagen according to manufacturer's protocol.
|
Label |
Hy5
|
Label protocol |
1 μg of total RNA from sample and reference was labelled with Hy3TM and Hy5TM respectively using miRCURYTM LNA Array power labelling kit from Exiqon according to the manufacturer's protocol.
|
|
|
|
Hybridization protocol |
The Hy3TM and Hy5TM labelled samples were mixed pairwise and hybridization was performed according to the miRCURYTM LNA array manual (Exiqon) using a Tecan HS4800 hybridization station
|
Scan protocol |
Microarray slides were scanned using the Agilent G2565BA Microarray Scanner System. Image Analysis used ImaGene 8.0 software.
|
Description |
raw data file Hy3: 1_Exiqon_13978199_S01_Cropped.txt raw data file Hy5: 0_Exiqon_13978199_S01_Cropped.txt Biological Replicate 6 of 7 for BALB WT mice
|
Data processing |
LOWESS normalized and background corrected (Normexp with offset value 10)
|
|
|
Submission date |
Dec 23, 2009 |
Last update date |
Jun 01, 2011 |
Contact name |
Christina Kathleen Haston |
E-mail(s) |
christina.haston@mcgill.ca
|
Phone |
514-398-3864 089714
|
Organization name |
McGill University
|
Department |
Medicine
|
Street address |
3626 St. Urbain
|
City |
Montreal |
State/province |
QC |
ZIP/Postal code |
H2X 2P2 |
Country |
Canada |
|
|
Platform ID |
GPL7723 |
Series (1) |
GSE19621 |
MicroRNA profiling of cystic fibrosis intestinal disease in mice |
|