|
Status |
Public on Dec 23, 2010 |
Title |
blood tissue from GDM woman |
Sample type |
RNA |
|
|
Source name |
GDM blood tissue
|
Organism |
Homo sapiens |
Characteristics |
tissue: blood
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA of whole blood was obtained using QIAamp RNA Blood Mini Kit (Qiagen) and digested with RNase-Free DNase (Promega) according to the manufacturer’s suggestions. Total RNA of the placenta tissue was obtained using the TRIzol reagent (Invitrogen) according to the manufacturer’s suggestions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays by using 500ng total RNA
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
n/a
|
Data processing |
The data were normalised using cubic spline method with Beadstudio software
|
|
|
Submission date |
Dec 24, 2009 |
Last update date |
Dec 23, 2010 |
Contact name |
Dapeng Wang |
Organization name |
Beijing Institute of Genomics
|
Street address |
No.7 Beitucheng West Road, Chaoyang District, Beijing 100029, PR China
|
City |
Beijing |
ZIP/Postal code |
100029 |
Country |
China |
|
|
Platform ID |
GPL7350 |
Series (1) |
GSE19649 |
Genomic expression profiles of blood and placenta in Chinese women with gestational diabetes |
|