|
| Status |
Public on Dec 23, 2010 |
| Title |
pooled blood tissues from four healthy women |
| Sample type |
RNA |
| |
|
| Source name |
non-GDM blood tissue
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of whole blood was obtained using QIAamp RNA Blood Mini Kit (Qiagen) and digested with RNase-Free DNase (Promega) according to the manufacturer’s suggestions. Total RNA of the placenta tissue was obtained using the TRIzol reagent (Invitrogen) according to the manufacturer’s suggestions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays by using 500ng total RNA
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
n/a
|
| Data processing |
The data were normalised using cubic spline method with Beadstudio software
|
| |
|
| Submission date |
Dec 24, 2009 |
| Last update date |
Dec 23, 2010 |
| Contact name |
Dapeng Wang |
| Organization name |
Beijing Institute of Genomics
|
| Street address |
No.7 Beitucheng West Road, Chaoyang District, Beijing 100029, PR China
|
| City |
Beijing |
| ZIP/Postal code |
100029 |
| Country |
China |
| |
|
| Platform ID |
GPL7350 |
| Series (1) |
| GSE19649 |
Genomic expression profiles of blood and placenta in Chinese women with gestational diabetes |
|
