|
| Status |
Public on Nov 13, 2023 |
| Title |
progression_CRLM8_1 |
| Sample type |
RNA |
| |
|
| Source name |
human leiomyoma
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: cadmium-treated
Sex: female
cell type: human uterine leiomyoma cell line immortalized via retroviral transfection of telemorase (ht-UtLM)
|
| Treatment protocol |
For 8-week Cd-exposure experiment, the ht-UtLM cultures were maintained continuously in ht-UtLM culture medium supplemented with 10 μM CdCl2 for 8 weeks. The culture medium was replaced with fresh medium with 10 μM CdCl2 every 3 days. Passage-matched control ht-UtLM cultures were run concurrently with Cd exposed cells. All cultures were kept in a standard tissue culture incubator at 37°C with 5% CO2.
|
| Growth protocol |
A human uterine leiomyoma cell line immortalized via retroviral transfection of telemorase (ht-UtLM) was generated by our laboratory. Human fibroblast and vulvar leiomyosarcoma (SKS-LMS-1) cell lines were cultured in DMEM medium supplemented with 10% FBS. All cultures were kept in a standard tissue culture incubator at 37°C with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by extracting with Trizol ™ Reagent (ThermoFisher Cat# 15596026) and subsequently purifying with an Rneasy Mini Kit (Qiagen Cat#74104).
|
| Label |
na
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
Reporter and Capture probes were added according to the manufacturer’s directions (nanostring.com) to 50 ng of total RNA in a final volume of 15ul followed by hybridization for at least 14 hours at 65oC
|
| Scan protocol |
After sample processing according to manufacturer’s instructions on the nCounter Prep Station Model 5S, sample cartridges were scanned on an nCounter Digital Analyzer Model 5S, using 555 Fields of Vision (FOV) and the .rlf file 'NS_Cancerprog_C4972'.
|
| Description |
Immortalized human leiomyoma cells, female, benign tumor, age unknown.
|
| Data processing |
Data were adjusted utilizing the manufacturer's positive and negative control probes, as well as internal housekeeping genes. This nSolver adjusted data were then imported into Partek(v7.0), log2 transformed and quantile normalized.
|
| |
|
| Submission date |
Nov 13, 2020 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Rick David Fannin |
| E-mail(s) |
fannin@niehs.nih.gov
|
| Phone |
9842874069
|
| Organization name |
Mr.
|
| Department |
NIEHS/NIH
|
| Lab |
Genomics Core
|
| Street address |
111 Tw Alexander Dr, MD D204
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platform ID |
GPL26599 |
| Series (2) |
| GSE161454 |
The effects of the prolonged Cd exposure on gene expression changes in in Uterine Leiomyoma Cells [cancer progression] |
| GSE161456 |
The effects of the prolonged Cd exposure on gene expression changes in in Uterine Leiomyoma Cells |
|
