|
| Status |
Public on Sep 01, 2010 |
| Title |
WI_Grotewold_091907 |
| Sample type |
genomic |
| |
|
| Source name |
Input DNA from wild type (Col) shoots (rep 3)
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
age: Ten-day old Green tissue
tissue: Shoots
antibody: none
|
| Growth protocol |
Ten-days old plants were grown under 16 h light on 1/2 MS plates
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
sonicated Arabidopsis genomic DNA
|
| Label |
biotin
|
| Label protocol |
The sample was 3'-end-labelled with dd-UTP (Roche) by the terminal transferase (TdT, Roche) reaction for 1.5 h at 37 degrees C.
|
| |
|
| Hybridization protocol |
Whole Genome Tiling Array was hybridized for 16 hrs, washed, and stained with a streptavidin-phycoerythrin conjugate on the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol.
|
| Scan protocol |
GeneChips were scanned on Affymetrix scanner G7
|
| Description |
none
|
| Data processing |
R Bioconductor was used to quantile normalize our ChIP-chip data
|
| |
|
| Submission date |
Jan 06, 2010 |
| Last update date |
Sep 04, 2019 |
| Contact name |
Zidian Xie |
| E-mail(s) |
xzd482@yahoo.com
|
| Organization name |
The Ohio State University
|
| Department |
Dept. of Plant Cellular and Molecular Biology
|
| Lab |
218 Rightmire hall
|
| Street address |
1060 Carmack Rd.
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43210 |
| Country |
USA |
| |
|
| Platform ID |
GPL10977 |
| Series (1) |
| GSE19763 |
Genome-wide FLP/MYB88 binding sites in Arabidopsis thaliana |
|
| Relations |
| Reanalyzed by |
GSE136843 |
