|
| Status |
Public on Feb 01, 2010 |
| Title |
mouse myocyte - Sanguinarine, 25 ug/ml-5 |
| Sample type |
RNA |
| |
|
| Source name |
mouse myocyte - Sanguinarine, 25 ug/ml
|
| Organism |
Mus musculus |
| Characteristics |
cell type: primary mouse myocytes
|
| Biomaterial provider |
Bioreach
|
| Treatment protocol |
Cells were grown to 85% confluency, treated for 60 minutes with vehicle (EtOH) or Sanguinarine at 1.5 ug/ml or 25 ug/ml. Total RNA extracted
|
| Growth protocol |
Standard growth media
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were washed with cold PBS and lysed with RNeasy (Quiagen, CA) and RNA was purified on a Quiagen Mini spin column.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol, 200 ng total RNA
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Standard Illumina protocol
|
| Description |
4658547081_B
|
| Data processing |
Normalized data with quantile normalization in Genome Studio v1.0. Raw data are not background subtracted.
|
| |
|
| Submission date |
Jan 07, 2010 |
| Last update date |
Jan 07, 2010 |
| Contact name |
David S Loose |
| E-mail(s) |
David.S.Loose@uth.tmc.edu
|
| Phone |
713-500-7440
|
| Organization name |
University of Texas
|
| Department |
Integrative Biology & Pharm
|
| Street address |
6431 Fannin St, MSB 4.130
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE19781 |
Effects of sanguinarine on murine myocyte gene expression |
|
