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| Status |
Public on Apr 20, 2021 |
| Title |
RNA sequencing Cat1-Spleen biological replicate 1 |
| Sample type |
SRA |
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| Source name |
Spleen
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| Organism |
Felis catus |
| Characteristics |
breed: A native breed of cat from Chengdu city
tissue: Spleen
age: 2-year-old
dev_stage: Adult
Sex: female
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
We used an rRNA depletion protocol (Ribo-Zero kit, Epicentre) coupled with the Illumina TruSeq stranded RNA-Seq library protocol to construct the RNA-Seq libraries. All libraries were quantified using the Qubit dsDNA High Sensitivity Assay Kit (Invitrogen) and sequenced on HiSeq X Ten (Illumina) platform to produce average ~ 49 million 150-bp paired-end raw reads and ~ 48 million high-quality reads for each library.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
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| Description |
Cat1-Spleen
cat_exp.txt
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| Data processing |
Gene-level expression was estimated as transcripts per million (TPM) using the high-speed transcript quantification tool Kallisto (version 0.43.0).
Genome_build: Felis_catus_6.2, Gallus_gallus-5.0, CanFam3.1, Cavpor3.0, Mmul_8.0.1, GRCm38.p5, OryCun2.0, Rnor_6.0, Oar_v3.1
Supplementary_files_format_and_content: genes expression level (TPM) text file
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| Submission date |
Nov 25, 2020 |
| Last update date |
Apr 20, 2021 |
| Contact name |
jin long |
| E-mail(s) |
longjin8806@163.com
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| Organization name |
Sichuan Agricultural University
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| Street address |
Hui ming
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| City |
Chengdu |
| ZIP/Postal code |
611130 |
| Country |
China |
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| Platform ID |
GPL29444 |
| Series (2) |
| GSE162142 |
A Pig BodyMap Transcriptome Reveals Diverse Tissue Physiologies and Evolutionary Dynamics of Transcription [RNA-Seq across species] |
| GSE162148 |
A Pig BodyMap Transcriptome Reveals Diverse Tissue Physiologies and Evolutionary Dynamics of Transcription |
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| Relations |
| BioSample |
SAMN15150104 |
| SRA |
SRX8483696 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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