|
| Status |
Public on Apr 20, 2021 |
| Title |
RNA-seq data of Pig replicate 4 sequenced by HiSeq X-ten |
| Sample type |
SRA |
| |
|
| Source name |
Adipose tissue
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Bama
tissue: Back fat/Upper layer of backfat
age: 2-year-old
developmental stage: Adult
Sex: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen).
We used an rRNA depletion protocol (Ribo-Zero kit, Epicentre) coupled with the Illumina TruSeq stranded RNA-Seq library protocol to construct the RNA-Seq libraries. All libraries were quantified using the Qubit dsDNA High Sensitivity Assay Kit (Invitrogen) and sequenced on HiSeq X Ten (Illumina) platform.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
TPM.pig.txt
BM-BC7-BPQ-LNC
|
| Data processing |
Gene-level expression was estimated as transcripts per million (TPM) using the high-speed transcript quantification tool Kallisto (version 0.43.0).
Genome_build: Rnor_6.0, Sscrofa11.1
Supplementary_files_format_and_content: genes expression level (TPM) text file
|
| |
|
| Submission date |
Nov 25, 2020 |
| Last update date |
Apr 20, 2021 |
| Contact name |
jin long |
| E-mail(s) |
longjin8806@163.com
|
| Organization name |
Sichuan Agricultural University
|
| Street address |
Hui ming
|
| City |
Chengdu |
| ZIP/Postal code |
611130 |
| Country |
China |
| |
|
| Platform ID |
GPL22918 |
| Series (2) |
| GSE162146 |
A Pig BodyMap Transcriptome Reveals Diverse Tissue Physiologies and Evolutionary Dynamics of Transcription [RNA-Seq for pig and rat] |
| GSE162148 |
A Pig BodyMap Transcriptome Reveals Diverse Tissue Physiologies and Evolutionary Dynamics of Transcription |
|
| Relations |
| BioSample |
SAMN15870132 |
| SRA |
SRX9002167 |
