|
| Status |
Public on Feb 26, 2021 |
| Title |
ECs normoxia1 |
| Sample type |
SRA |
| |
|
| Source name |
Lung
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Endothelial cells (ECs)
strain: Sprague-Dawley
genotype: Wild type
treatment: Normoxia
days: 4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells and tissues were lysed in Trizol and RNA was extracted using PureLink RNA Mini Kit (Thermo Fisher Scientific,) and PureLink DNase (Thermo Fisher Scientific,) according to manufacturer’s instructions.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
A minimum 25M reads per library with 75 bp paired-ends reads were generated per sample.
Quality control of the resulting sequence data was performed using FastQC
Trimmed and filtered reads were aligned to rat genome version rn6 reference
Genome_build: Rn6
Supplementary_files_format_and_content: Matrix table with FPKM values for every gene and every sample
|
| |
|
| Submission date |
Nov 27, 2020 |
| Last update date |
Feb 26, 2021 |
| Contact name |
Takeshi Masaki |
| E-mail(s) |
tmasaki@ncvc.go.jp
|
| Phone |
81-6-6170-1070
|
| Organization name |
National Cerebral and Cardiovascular Center
|
| Department |
Vascular Physiology
|
| Street address |
6-1
|
| City |
Kishibe-Shinmachi,Suita |
| State/province |
Osaka |
| ZIP/Postal code |
564-8565 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20084 |
| Series (2) |
| GSE162239 |
Aryl hydrocarbon receptor is essential for the pathogenesis of pulmonary arterial hypertension [day 4 RNA-Seq] |
| GSE162245 |
RNA-seq analysis of Ahr-knockout rats of SuHx model. |
|
| Relations |
| BioSample |
SAMN16935510 |
| SRA |
SRX9596865 |
