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| Status |
Public on Dec 02, 2020 |
| Title |
X1D30-3 |
| Sample type |
SRA |
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| Source name |
brain tissue
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| Organism |
Apis mellifera ligustica |
| Characteristics |
tissue: brain
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| Extracted molecule |
total RNA |
| Extraction protocol |
Brain tissue were collected,and total RNA was extracted by Trizol reagent kit (Invitrogen, Carlsbad, CA,USA). Sequenced using Illumina HiSeq TM 2500 by Gene Denovo Biotechnology Co.
RNA libraries were prepared for sequencing using standard Illumina protocols
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| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2500 |
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| Data processing |
Illumina HiSeq TM 2500 by Gene Denovo Biotechnology Co
To get clean tags,sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence.
All of the clean tags were aligned with small RNAs in GeneBank database (Release 209.0) and Rfam database (Release11.0) to identify and remove rRNA,scRNA,snoRNA,snRNA and tRNA.Then aligned with Genome to remove introns and repeat sequences.
Identification of exist miRNA,known miRNA and novel miRNA with miRbase database (Release 2).
Differentially expressed miRNA (DE miRNA) analysis and target gene prediction
Supplementary_files_format_and_content: tab-delimited text files include miRNA expression for each Sample.
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| Submission date |
Dec 01, 2020 |
| Last update date |
Dec 02, 2020 |
| Contact name |
Chao Tianle |
| E-mail(s) |
chaotianle@sdau.edu.cn
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| Phone |
15153876133
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| Organization name |
6/5000 Shandong Agricultural University
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| Street address |
Daizong street, no. 61, 6/5000 Shandong Agricultural University
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| City |
Tai’an |
| State/province |
Shandong |
| ZIP/Postal code |
538 |
| Country |
China |
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| Platform ID |
GPL24122 |
| Series (1) |
| GSE162423 |
MicroRNA expression profile of Apis mellifera ligustica brain tissue |
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| Relations |
| BioSample |
SAMN16969600 |
| SRA |
SRX9613417 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM4951870_X1D30-3_exp.txt.gz |
18.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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