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Sample GSM496132 Query DataSets for GSM496132
Status Public on Jan 12, 2011
Title liver-CCL4-8weeks-rep2
Sample type RNA
 
Source name Liver, 8 weeks, CCL4, replicate 2
Organism Mus musculus
Characteristics tissue: Liver
time: 8 weeks
administrated reagent: CCL4
disease state: fibrosis
Treatment protocol CCL4 or olive oil was administrated intraperitoneally in mouse twice a week for 4 weeks and then administrated once a week for four weeks. Mice were sacrificed on 4, 6, and 8 weeks.
Extracted molecule total RNA
Extraction protocol Total RNA extracted using mirVana miRNA isolation kit following manufacturer's instructions
Label Cy3
Label protocol miRNA microarrays were manufactured by Agilent Technologies, and 100 ng of total RNA was labeled and hybridized using Mouse microRNA Microarray Kit according to manufacture’s protocol (Protocol for use with Agilent microRNA microarrays Version 1.0)
 
Hybridization protocol miRNA microarrays were manufactured by Agilent Technologies, and 100 ng of total RNA was labeled and hybridized using Mouse microRNA Microarray Kit according to manufacture’s protocol (Protocol for use with Agilent microRNA microarrays Version 1.0)
Scan protocol Scanned on an Agilent G2505B scanner
Description miRNA expression after 8 weeks in CCL4-administrated mouse liver
Data processing Data were analyzed using GeneSpring GX 7.3.1 software (Agilent Technologies) and normalized as follows: (i) Values below 0.01 were set to 0.01. (ii) Each measurement was divided by the 75th percentile of all measurements to compare one-color expression profiles.
 
Submission date Jan 12, 2010
Last update date Jan 12, 2011
Contact name Nobuyoshi Kosaka
E-mail(s) nkosaka@ncc.go.jp
Phone +81-3-3542-2511
Fax +81-3-5565-0727
Organization name National Cancer Center Research Institute
Department Section for Studies on Metastasis
Street address 5-1-1, Tsukiji, Chuo-ku
City Tokyo
ZIP/Postal code 104-0045
Country Japan
 
Platform ID GPL8824
Series (1)
GSE19865 Aberrant expression of microRNAs is associated to the progression of liver fibrosis

Data table header descriptions
ID_REF
VALUE Normalized, log2 signal intensity

Data table
ID_REF VALUE
miRNABrightCorner30 10.270622
DarkCorner 4.3025455
mghv-miR-M1-7-3p 2.6908662
mmu-miR-471 0
mmu-miR-671-3p 0
mmu-miR-384-3p 0
mmu-miR-197 3.0265434
mmu-miR-27a* 0
mmu-miR-678 0
mmu-miR-96 4.6884265
mmu-miR-297b-5p 0
mmu-miR-26b 9.493989
mmu-miR-709 8.134195
mmu-miR-693-3p 0
mmu-miR-196b 1.7858603
mmu-let-7e 7.233706
mmu-miR-700 0.114833385
mmu-miR-362-5p 0
mmu-miR-468 0
mmu-miR-138 0

Total number of rows: 599

Table truncated, full table size 10 Kbytes.




Supplementary file Size Download File type/resource
GSM496132.txt.gz 1.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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