|
| Status |
Public on Jul 01, 2011 |
| Title |
RatSpinalCord-Sham 3 day-2 |
| Sample type |
RNA |
| |
|
| Source name |
spinal cord, sham, 3 days after surgery
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue type: spinal cord
gender: female
strain: Wistar
treatment: Sham
time point: 3 days after surgery
|
| Treatment protocol |
Controls: no treatment; Injuried: laminectomy and 200 Kdynes contusion injury at T8 level; Sham: laminectomy at T8 level but no contussion. Contussions using Contusor IH Spinal Cord Impactor (Precision System & Instrumentation, LLC, Va)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAlater (Qiagen) preservation followed by miRNeasy Mini kit (Qiagen) extraction
|
| Label |
Hy3
|
| Label protocol |
Using the reactives and protocols included in the miRCURY LNA™ microRNA Power labeling kit, Hy3 dye.
|
| |
|
| Hybridization protocol |
Following the Exiqon protocols included in the miRCURY LNA™ microRNA Array kit v.11.0
|
| Scan protocol |
Arrays were scanned by the Genomic Service of the Madrid Science Park using a GenePix4000B scanner, the GenePix Pro 5.0.1.24 software, and the specific GAL file for the miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno. Scanner settings are detailed in every raw data file.
|
| Description |
SH3X584
|
| Data processing |
Data provided in the matrix spreadsheet was normalized following the Variance Stabilization Method developed by Huber and developed in R language by the Bioconductor facility.
|
| |
|
| Submission date |
Jan 14, 2010 |
| Last update date |
Jul 01, 2011 |
| Contact name |
Rodrigo M Maza |
| E-mail(s) |
rodrigom@sescam.jccm.es
|
| Organization name |
Hospital Nacional de Parapléjicos (SESCAM)
|
| Department |
Unidad de Neurología Experimental
|
| Lab |
Molecular Neuroprotection Lab
|
| Street address |
Finca La Peraleda s/n
|
| City |
Toledo |
| State/province |
Toledo |
| ZIP/Postal code |
45071 |
| Country |
Spain |
| |
|
| Platform ID |
GPL9908 |
| Series (1) |
| GSE19890 |
miRNA expression after moderate Spinal Cord Injury in Rats |
|
