|
| Status |
Public on Dec 31, 2010 |
| Title |
Pt 2_Ballif et al._17q23.2 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
test genomic DNA, extracted from blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
test: Patient 2
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from lymphoblastoid cell lines, peripheral blood, or cultured tissues of the subjects and phenotypically normal male and female references using MagAttract DNA Mini M48 following the manufacture's instructions.
|
| Label |
AF5
|
| Label protocol |
500 ng of sonicated DNA was labeled with AF5 (patient) and AF3 (control) using BioPrime Total labeling kit (Invitrogen) per manufacturer's instructions
|
| |
|
| Channel 2 |
| Source name |
control genomic DNA, extracted from blood
|
| Organism |
Homo sapiens |
| Characteristics |
gender: Female
reference: phenotypically normal female
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from lymphoblastoid cell lines, peripheral blood, or cultured tissues of the subjects and phenotypically normal male and female references using MagAttract DNA Mini M48 following the manufacture's instructions.
|
| Label |
AF3
|
| Label protocol |
500 ng of sonicated DNA was labeled with AF5 (patient) and AF3 (control) using BioPrime Total labeling kit (Invitrogen) per manufacturer's instructions
|
| |
|
| |
| Hybridization protocol |
Hybridization and washing were perfomed with Agilent reagents per manufacturer's instructions
|
| Scan protocol |
Scanned on Axon 4000B scanner (Molecular Devices)
|
| Description |
n/a
|
| Data processing |
Agilent Feature Extraction software v9.5.3.1 and Agilent CGH Analytics software v3.5.14 and displayed using oligonucleotide aCGH analysis software Genoglyphix
|
| |
|
| Submission date |
Jan 19, 2010 |
| Last update date |
Dec 31, 2010 |
| Contact name |
Blake C. Ballif |
| Organization name |
Signature Genomic Laboratories
|
| Department |
Research & Development
|
| Street address |
2820 North Astor Street
|
| City |
Spokane |
| State/province |
WA |
| ZIP/Postal code |
99207 |
| Country |
USA |
| |
|
| Platform ID |
GPL4091 |
| Series (1) |
| GSE19932 |
Recurrent 17q23.2 microdeletion flanked by segmental duplications associated with heart defects and limb abnormalities |
|
