|
| Status |
Public on Dec 01, 2023 |
| Title |
Mbl8MM_day3_2_181105_L7_S5 |
| Sample type |
SRA |
| |
|
| Source name |
Mbl8MM_control5
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: Actin-GAL4 UAS-sh8MM
tissue: brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Brains were disected in cold PBS. Total RNA was extracted using Trizol reagent (Sigma) and treated with DNase I (NEB) following the manufacturer's protocol.
Cell-seq2 library preparation was done as in Hashimshony et al 2016, adapted for bulk RNA.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Data was aligned using STAR to Drosophila melanogaster genome version dm6
Counting number of reads for each gene at the 3' end using ESAT tool (Derr A. et al 2016)
Genome_build: dm6
Supplementary_files_format_and_content: normalized read counts
|
| |
|
| Submission date |
Dec 23, 2020 |
| Last update date |
Dec 01, 2023 |
| Contact name |
Sebastian Kadener |
| E-mail(s) |
skadener@brandeis.edu
|
| Organization name |
Brandeis University
|
| Department |
Biology department
|
| Street address |
415 South Street
|
| City |
Waltham |
| State/province |
MA |
| ZIP/Postal code |
02453 |
| Country |
USA |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE163797 |
3' RNA-seq of brains from control and circMbl KD flies |
|
| Relations |
| BioSample |
SAMN17150118 |
| SRA |
SRX9726998 |
