|
Status |
Public on Dec 25, 2023 |
Title |
C3_HCT8 with hypoxia treatment |
Sample type |
SRA |
|
|
Source name |
HCT8 with hypoxia treatment
|
Organism |
Homo sapiens |
Characteristics |
cell line: HCT8 cell type: CRC cell lines passages: P5 treatment: hypoxia
|
Growth protocol |
All of the cells were cultured at 37°C in Dulbecco’s Modified Eagle Medium (DMEM; Gibco, Thermo Fisher Scientific, St Peters, MO, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, St Peters, MO, USA) in a 5% CO2 atmosphere.
|
Extracted molecule |
total RNA |
Extraction protocol |
prefrontal cortex were rharvested , flash frozen on dry ice, and RNA was harvested using Trizol reagent. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Description |
R20021475-NBC20200608-A-HCT8-hypoxia
|
Data processing |
We applied the reads filtering towards the raw reads after sequencing to achieve the clean data following the criteria: a)10% base quality <15 b) 13% base quality <20. Hisat2 2.0.4 mapping to reference genome count counting Genome_build: GRCh38 Supplementary_files_format_and_content: TXT file contain counts for each sample
|
|
|
Submission date |
Dec 25, 2020 |
Last update date |
Dec 25, 2023 |
Contact name |
Zhenxing Liang |
E-mail(s) |
1051322781@qq.com
|
Organization name |
The Sixth Affiliated Hospital, Sun Yat-sen University
|
Street address |
26 Yuancun Erheng Rd
|
City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510655 |
Country |
China |
|
|
Platform ID |
GPL20795 |
Series (1) |
GSE163868 |
Next Generation Sequencing of normal human intestinal epithelial cell lines, CRC cell lines and CRC cell lines with hypoxia treatment |
|
Relations |
BioSample |
SAMN17159354 |
SRA |
SRX9735410 |