|
| Status |
Public on Jul 01, 2021 |
| Title |
rnai_2 |
| Sample type |
RNA |
| |
|
| Source name |
RNA from fly heads, rnai only
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: rnai
tissue: head
|
| Treatment protocol |
Flies harboring the neuron-selective elav-Gal4 driver and/or Clic UAS-RNAi transgenev105975 were reared and crossed as previously described (Chan et al. 2014). Fly strains were raised and processed under identical conditions.
|
| Growth protocol |
n/a
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was obtained from whole fly head homogenates as described previously (Jones et al. 2014).
|
| Label |
biotin
|
| Label protocol |
Botinylated cRNA samples were prepared according to Affymetrix protocols.
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA samples were hybridized to GeneChip Drosophila Gene 1.0 ST arrays by procedures outlined by Affymetrix.
|
| Scan protocol |
Affymetrix Genechip scanner 3000 7G was used scanning and AGCC software for analysis, per manufacturer protocols.
|
| Description |
Gene expression data from whole fly heads tissue, rnai-only control strain
|
| Data processing |
Microarray CEL files were processed with the R package Oligo v1.44.0 for background subtraction and normalization, which implements the robust multi-array average (RMA) method.
|
| |
|
| Submission date |
Dec 31, 2020 |
| Last update date |
Jul 01, 2021 |
| Contact name |
Michael Miles |
| E-mail(s) |
Michael.Miles@vcuhealth.org
|
| Organization name |
Virginia Commonwealth Univ.
|
| Department |
Pharmacology and Toxicology
|
| Lab |
Miles
|
| Street address |
Box 980613
|
| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23298 |
| Country |
USA |
| |
|
| Platform ID |
GPL20842 |
| Series (1) |
| GSE164090 |
Chloride Intracellular Channel Knockdown in Drosophila Alters the Transcriptome, Sensitivity to Ethanol Sedation, and Oxidation-Reduction Processes |
|
