|
| Status |
Public on Jun 23, 2021 |
| Title |
GC0223 Gastric Cancer |
| Sample type |
RNA |
| |
|
| Source name |
Serum
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Gastric Cancer
Sex: Male
age: 52
Stage: IB
histological subtype: undiff
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted each from 300uL serum samples using 3D-Gene® RNA extraction reagent from liquid sample kit (Toray Industries, Inc.).
|
| Label |
Cy5
|
| Label protocol |
miRNA was labeled using 3D-Gene® miRNA Labeling kit in accordance with the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Hybridized for 16 h at 32 ºC with rotary shaker (250 rpm). Hybridization buffer and washing protocol was attached in the 3D-Gene® miRNA oligo chip kit.
|
| Scan protocol |
3D-Gene® Scanner (Toray Industries, Inc.) was used for scanning. Images were quantified using Extraction version 2.0.0.6 (Toray Industries, Inc.).
|
| Data processing |
The presence of miRNA was determined based on a corresponding microarray signal of greater than [the mean + 2 × standard deviation] of the negative controls signal, of which the top and bottom ranked ones by signal intensity were removed. Once a miRNA was considered present, the mean signal of the negative controls of which the top and bottom 5% ranked by signal intensity were removed was subtracted from the miRNA signal. When the signal value was negative (or undetected) after background subtraction, the value was replaced by 0.1 on a base 2 logarithm scale.
|
| |
|
| Submission date |
Jan 04, 2021 |
| Last update date |
Jun 23, 2021 |
| Contact name |
Satoko Takizawa |
| E-mail(s) |
satoko.takizawa.f3@mail.toray
|
| Organization name |
Toray industries inc
|
| Street address |
kamakurashi
|
| City |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21263 |
| Series (1) |
| GSE164174 |
A novel combination of serum microRNAs for the detection of early gastric cancer |
|
