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Status |
Public on Jan 05, 2021 |
Title |
006-ctr030: Peripheral Blood_Healthy control |
Sample type |
RNA |
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Source name |
Peripheral Blood
|
Organism |
Homo sapiens |
Characteristics |
gender: Female age: 42 disease status: normal tissue: Peripheral Blood
|
Treatment protocol |
Blood sample was treated according to the manusfacturer's instructions (PAXgene Blood RNA Tube)
|
Growth protocol |
Elbow vein blood sampling and the blood was stored at -20℃ before RNA extraction.
|
Extracted molecule |
total RNA |
Extraction protocol |
Peripheral blood of total RNA was performed according to the manufacturer's instructions (PAXgene Blood RNA kit).
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 200 ng total RNA (Technical Manual for GeneChip 3’IVT Expression Kit,2013, Affymetrix).
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Hybridization protocol |
Following fragmentation, 15 μg cRNA were hybridized for 16 hr at 45℃ on GeneChip 3’IVT Expression Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip® System 3000Dx (GCS 3000Dx).
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Description |
Gene expression data from healthy person with normal colonscopy
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Data processing |
The data were analyzed with Affymetrix Expression Console (ver.1.3.1) using Affymetrix default analysis settings and MAS5 global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
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Submission date |
Jan 04, 2021 |
Last update date |
Jan 05, 2021 |
Contact name |
changming cheng |
E-mail(s) |
cmcheng2005@gmail.com
|
Organization name |
National Engineering Center for Biochip at Shanghai China
|
Street address |
No. 151, Libing Road
|
City |
Shanghai |
ZIP/Postal code |
201203 |
Country |
China |
|
|
Platform ID |
GPL570 |
Series (1) |
GSE164191 |
Blood based expression of colorectal cancer and healthy controls |
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