|
| Status |
Public on Jan 26, 2010 |
| Title |
imiquimod1 |
| Sample type |
RNA |
| |
|
| Source name |
spontaneous mammary tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB/N-TgN (MMTVneu)-202Mul
treatment: imiquimod cream
|
| Treatment protocol |
topical imiquimod
|
| Growth protocol |
spontaneous tumors in neu transgenic mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from tumors using RNAqueous4PCR kit (Ambion)
|
| Label |
biotin
|
| Label protocol |
labeled cRNA were generated using the Illumina TotalPrep RNA Amplification kit from Ambion (cat# IL1791)
|
| |
|
| Hybridization protocol |
biotin-labeled cRNA were hybridized to Sentrix Mouse Ref-8 Expression Bead Chip v1.0a
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
Trimmed mean intensities were log2 transformed and quantile normalized using affy package from Bioconductor.org.
|
| |
|
| Submission date |
Jan 25, 2010 |
| Last update date |
Jan 25, 2010 |
| Contact name |
Lynn M Amon |
| E-mail(s) |
lynn.amon@cidresearch.org
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Division of Public Health Sciences
|
| Lab |
Computation Biology Program
|
| Street address |
1100 Fairview, M1-C108
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-1024 |
| Country |
USA |
| |
|
| Platform ID |
GPL9974 |
| Series (1) |
| GSE20032 |
Comparison of Genes in Imiquimod-treated and Control Tumors |
|
