|
| Status |
Public on Jan 27, 2010 |
| Title |
50% C2 blasts_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
C2C12 cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: C2C12
cell type: myoblast
confluence: 50%
|
| Growth protocol |
C2C12 myoblasts were cultured in DMEM supplemented with 10% BCS. If needed, 100% confluent myoblasts were induced to differentiate into myotubes in DMEM supplemented with 2% heat-inactive horse serum, 10ug/ml insulin, and 10ug/ml transferrin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 2
|
| Data processing |
The data were normalized and summarized using RMA in the lumi package of Bioconductor
|
| |
|
| Submission date |
Jan 27, 2010 |
| Last update date |
Jan 27, 2010 |
| Contact name |
Yi Cao |
| E-mail(s) |
ycao@fhcrc.org
|
| Phone |
206-667-5278
|
| Organization name |
Fred Hutchinson CRC
|
| Department |
Human Biology
|
| Lab |
Tapscott
|
| Street address |
1100 Fairview Ave. N
|
| City |
Seatttle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE20059 |
Genome-wide analysis of gene expression during differentiation in C2C12 cells |
|
