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Sample GSM5014661 Query DataSets for GSM5014661
Status Public on Sep 27, 2021
Title Car_CD34p_GFP
Sample type RNA
 
Source name CD34+ tumor cells from DMBA tumor
Organism Mus musculus
Characteristics tumor type: Carcinoma
Extracted molecule total RNA
Extraction protocol RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
Label biotin
Label protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
 
Hybridization protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Scan protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Description Gene expression data from Nr2f2 carcinoma
Data processing To normalize the data, the Robust Multi-array Average expression (RMA) method from the affy package was used.
 
Submission date Jan 11, 2021
Last update date Sep 27, 2021
Contact name Yura Song
E-mail(s) yura.song@ulb.be
Organization name Université Libre de Bruxelles
Lab Laboratory of Stem Cells and Cancer
Street address 808, route de Lennik
City Bruxelles
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL1261
Series (2)
GSE164597 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation II
GSE164605 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation

Data table header descriptions
ID_REF
VALUE log2-transformed intensity

Data table
ID_REF VALUE
1415670_at 9.912
1415671_at 10.410
1415672_at 11.555
1415673_at 8.035
1415674_a_at 8.720
1415675_at 8.633
1415676_a_at 11.348
1415677_at 8.311
1415678_at 10.621
1415679_at 11.046
1415680_at 9.799
1415681_at 8.878
1415682_at 7.922
1415683_at 11.121
1415684_at 7.592
1415685_at 7.617
1415686_at 9.667
1415687_a_at 11.969
1415688_at 10.760
1415689_s_at 9.357

Total number of rows: 45101

Table truncated, full table size 769 Kbytes.




Supplementary file Size Download File type/resource
GSM5014661_hyb6626.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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