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Sample GSM5014663 Query DataSets for GSM5014663
Status Public on Sep 27, 2021
Title Pap_CD34p_1
Sample type RNA
 
Source name CD34+ tumor cells from DMBA tumor
Organism Mus musculus
Characteristics tumor type: Papilloma
Extracted molecule total RNA
Extraction protocol RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
Label biotin
Label protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
 
Hybridization protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Scan protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Description Gene expression data from Nr2f2 Papilloma
Data processing To normalize the data, the Robust Multi-array Average expression (RMA) method from the affy package was used.
 
Submission date Jan 11, 2021
Last update date Sep 27, 2021
Contact name Yura Song
E-mail(s) yura.song@ulb.be
Organization name Université Libre de Bruxelles
Lab Laboratory of Stem Cells and Cancer
Street address 808, route de Lennik
City Bruxelles
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL1261
Series (2)
GSE164597 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation II
GSE164605 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation

Data table header descriptions
ID_REF
VALUE log2-transformed intensity

Data table
ID_REF VALUE
1415670_at 9.926
1415671_at 9.847
1415672_at 11.627
1415673_at 10.044
1415674_a_at 10.011
1415675_at 9.681
1415676_a_at 11.254
1415677_at 9.295
1415678_at 10.259
1415679_at 10.876
1415680_at 9.893
1415681_at 10.137
1415682_at 8.874
1415683_at 10.928
1415684_at 8.442
1415685_at 8.583
1415686_at 9.725
1415687_a_at 11.233
1415688_at 10.639
1415689_s_at 9.179

Total number of rows: 45101

Table truncated, full table size 769 Kbytes.




Supplementary file Size Download File type/resource
GSM5014663_hyb7337.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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