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Sample GSM5014664 Query DataSets for GSM5014664
Status Public on Sep 27, 2021
Title Car_CD34p_2
Sample type RNA
 
Source name CD34+ tumor cells from DMBA tumor
Organism Mus musculus
Characteristics tumor type: Carcinoma
Extracted molecule total RNA
Extraction protocol RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
Label biotin
Label protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
 
Hybridization protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Scan protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Description Gene expression data from Nr2f2 carcinoma
Data processing To normalize the data, the Robust Multi-array Average expression (RMA) method from the affy package was used.
 
Submission date Jan 11, 2021
Last update date Sep 27, 2021
Contact name Yura Song
E-mail(s) yura.song@ulb.be
Organization name Université Libre de Bruxelles
Lab Laboratory of Stem Cells and Cancer
Street address 808, route de Lennik
City Bruxelles
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL1261
Series (2)
GSE164597 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation II
GSE164605 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation

Data table header descriptions
ID_REF
VALUE log2-transformed intensity

Data table
ID_REF VALUE
1415670_at 10.411
1415671_at 9.644
1415672_at 11.676
1415673_at 10.190
1415674_a_at 9.825
1415675_at 9.435
1415676_a_at 11.214
1415677_at 9.283
1415678_at 10.187
1415679_at 11.049
1415680_at 9.856
1415681_at 9.989
1415682_at 8.699
1415683_at 10.855
1415684_at 8.190
1415685_at 8.259
1415686_at 9.568
1415687_a_at 11.169
1415688_at 10.553
1415689_s_at 9.225

Total number of rows: 45101

Table truncated, full table size 769 Kbytes.




Supplementary file Size Download File type/resource
GSM5014664_hyb7339.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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