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Sample GSM5014666 Query DataSets for GSM5014666
Status Public on Sep 27, 2021
Title Car_CD34p_3
Sample type RNA
 
Source name CD34+ tumor cells from DMBA tumor
Organism Mus musculus
Characteristics tumor type: Carcinoma
Extracted molecule total RNA
Extraction protocol RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
Label biotin
Label protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
 
Hybridization protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Scan protocol Microarray analysis was performed at the VIB MicroArray Facility in Leuven. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Description Gene expression data from Nr2f2 carcinoma
Data processing To normalize the data, the Robust Multi-array Average expression (RMA) method from the affy package was used.
 
Submission date Jan 11, 2021
Last update date Sep 27, 2021
Contact name Yura Song
E-mail(s) yura.song@ulb.be
Organization name Université Libre de Bruxelles
Lab Laboratory of Stem Cells and Cancer
Street address 808, route de Lennik
City Bruxelles
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL1261
Series (2)
GSE164597 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation II
GSE164605 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation

Data table header descriptions
ID_REF
VALUE log2-transformed intensity

Data table
ID_REF VALUE
1415670_at 9.985
1415671_at 9.309
1415672_at 11.658
1415673_at 9.292
1415674_a_at 9.405
1415675_at 8.888
1415676_a_at 11.036
1415677_at 8.788
1415678_at 10.329
1415679_at 10.805
1415680_at 9.607
1415681_at 9.528
1415682_at 8.050
1415683_at 10.517
1415684_at 7.210
1415685_at 8.690
1415686_at 9.154
1415687_a_at 11.225
1415688_at 10.064
1415689_s_at 8.646

Total number of rows: 45101

Table truncated, full table size 769 Kbytes.




Supplementary file Size Download File type/resource
GSM5014666_hyb8371.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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