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Sample GSM5014747 Query DataSets for GSM5014747
Status Public on Sep 27, 2021
Title LOF_Control1
Sample type RNA
 
Source name Ctrl carcinoma 1
Organism Mus musculus
Characteristics tumor type: Carcinoma
genotype: NR2F2 WT
Extracted molecule total RNA
Extraction protocol RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
Label biotin
Label protocol Microarray analysis was performed at the IRB Functional Genomics Core, Barcelona. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
 
Hybridization protocol Microarray analysis was performed at the IRB Functional Genomics Core, Barcelona. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Scan protocol Microarray analysis was performed at the IRB Functional Genomics Core, Barcelona. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Description Gene expression data from Nr2f2 control corresponding to LOF
Data processing To normalize the data, the Robust Multi-array Average expression (RMA) method from the affy package was used.
 
Submission date Jan 11, 2021
Last update date Sep 27, 2021
Contact name Yura Song
E-mail(s) yura.song@ulb.be
Organization name Université Libre de Bruxelles
Lab Laboratory of Stem Cells and Cancer
Street address 808, route de Lennik
City Bruxelles
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL11180
Series (2)
GSE164602 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation III
GSE164605 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation

Data table header descriptions
ID_REF
VALUE log2-transformed intensity

Data table
ID_REF VALUE
1415670_PM_at 10.7739
1415671_PM_at 10.3646
1415672_PM_at 9.5399
1415673_PM_at 8.2837
1415674_PM_a_at 9.0741
1415675_PM_at 8.7390
1415676_PM_a_at 10.9523
1415677_PM_at 10.2916
1415678_PM_at 10.2883
1415679_PM_at 10.7306
1415680_PM_at 9.0572
1415681_PM_at 8.9180
1415682_PM_at 7.7308
1415683_PM_at 9.3930
1415684_PM_at 5.9997
1415685_PM_at 7.5043
1415686_PM_at 9.3725
1415687_PM_a_at 10.7231
1415688_PM_at 8.0102
1415689_PM_s_at 7.0587

Total number of rows: 45141

Table truncated, full table size 946 Kbytes.




Supplementary file Size Download File type/resource
GSM5014747_1667_2017.ga.cel.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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