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Sample GSM5014749 Query DataSets for GSM5014749
Status Public on Sep 27, 2021
Title LOF1
Sample type RNA
 
Source name LOF carcinoma 1
Organism Mus musculus
Characteristics tumor type: Carcinoma
genotype: NR2F2 KO
Extracted molecule total RNA
Extraction protocol RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
Label biotin
Label protocol Microarray analysis was performed at the IRB Functional Genomics Core, Barcelona. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
 
Hybridization protocol Microarray analysis was performed at the IRB Functional Genomics Core, Barcelona. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Scan protocol Microarray analysis was performed at the IRB Functional Genomics Core, Barcelona. cDNA synthesis, library preparation and amplification were performed as described in Gonzalez-Roca, E. et al., 2010. A Mouse Genome 430pm strip Affymetrix array was used and the data were normalized using RMA algorithm
Description Gene expression data from Nr2f2 LOF
Data processing To normalize the data, the Robust Multi-array Average expression (RMA) method from the affy package was used.
 
Submission date Jan 11, 2021
Last update date Sep 27, 2021
Contact name Yura Song
E-mail(s) yura.song@ulb.be
Organization name Université Libre de Bruxelles
Lab Laboratory of Stem Cells and Cancer
Street address 808, route de Lennik
City Bruxelles
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL11180
Series (2)
GSE164602 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation III
GSE164605 NR2F2 controls malignant squamous cell carcinoma state by promoting stemness and invasion and repressing differentiation

Data table header descriptions
ID_REF
VALUE log2-transformed intensity

Data table
ID_REF VALUE
1415670_PM_at 10.6377
1415671_PM_at 10.1364
1415672_PM_at 9.4066
1415673_PM_at 9.3028
1415674_PM_a_at 8.8370
1415675_PM_at 8.5430
1415676_PM_a_at 11.1725
1415677_PM_at 10.0806
1415678_PM_at 10.2338
1415679_PM_at 10.4864
1415680_PM_at 9.1408
1415681_PM_at 8.8882
1415682_PM_at 8.5248
1415683_PM_at 9.6219
1415684_PM_at 5.9618
1415685_PM_at 7.1431
1415686_PM_at 9.4875
1415687_PM_a_at 10.6039
1415688_PM_at 8.2495
1415689_PM_s_at 6.5872

Total number of rows: 45141

Table truncated, full table size 946 Kbytes.




Supplementary file Size Download File type/resource
GSM5014749_1670_2017.ga.cel.gz 2.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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