|
| Status |
Public on Apr 25, 2010 |
| Title |
HeLa_Pol3_ChIPSeq |
| Sample type |
SRA |
| |
|
| Source name |
HeLa, ChIP_Seq
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
chip antibody: anti-PolIII
|
| Growth protocol |
HeLa cells were maintained in Dulbeccos modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum and 1 mM glutamine.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
For chromatin immunoprecipitation (ChIP) using antibodies for PolIII, the formaldehyde cross-linked cells were sonicated to obtain DNA fragments ranging in size from ~200 bp. Cluster generation and sequencing were performed as described earlier (Barski et al Cell, 2007) .
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
Chromatin IP against Pol III (RPC155)
|
| Data processing |
Illumina Genome Analyzer Pipeline produced short sequence reads that were matched to the hg18 human genome using ELAND alignment algorithm.
|
| |
|
| Submission date |
Jan 27, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Iouri Chepelev |
| E-mail(s) |
ichepelev@gmail.com
|
| Organization name |
US Department of Veterans Affairs Medical Center
|
| Street address |
3200 Vine St
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45220 |
| Country |
USA |
| |
|
| Platform ID |
GPL9115 |
| Series (2) |
| GSE20040 |
Pol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes (I) |
| GSE20072 |
Pol II and its associated epigenetic marks are present at pol III-transcribed non-coding RNA genes |
|
| Relations |
| SRA |
SRX018606 |
| BioSample |
SAMN00010735 |
| Named Annotation |
GSM501710_HeLa_Pol3.bed.gz |
