NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM502338 Query DataSets for GSM502338
Status Public on Jul 01, 2010
Title MEF HDAC1 fl/fl + siRNA scrambled + EtOH rep3
Sample type RNA
 
Source name MEF HDAC1 fl/fl + siRNA scrambled + EtOH
Organism Mus musculus
Characteristics cells: primary MEF
gender: Male
genotype: WT
Treatment protocol cells were treatment with either Ethanol or Tamoxifen at 200 nanoMolar
Growth protocol MEF cultures were grown at 37°C in Dulbecco’s-modified Eagle’s medium supplemented with 10% heat-inactivated FBS and split 1:3.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Minikit (Qiagen) according to the manufacturer’s protocol.
Label biotin
Label protocol 1-cycle IVT protocol for Affymetrix Mouse 430v2GeneChips
 
Hybridization protocol standard Affymetrix procedure
Scan protocol standard Affymetrix precedure
Description gene expression data from WT cells
Data processing Affymetrix and Expressionist
 
Submission date Jan 29, 2010
Last update date Jan 29, 2010
Contact name Patrick Matthias
E-mail(s) patrick.matthias@fmi.ch
Organization name Friedrich Miescher Institute for Biomed. Research
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platform ID GPL1261
Series (1)
GSE20100 Expression data from primary MEF lacking either HDAC1, HDAC2 or both

Data table header descriptions
ID_REF
VALUE RMA normalized data

Data table
ID_REF VALUE
1448595_a_at 21.285805
1423327_at 5484.946
1435275_at 58.939167
1449009_at 26.255836
1429159_at 17.834417
1449368_at 22.328178
1427168_a_at 14.307636
1421965_s_at 20.229866
1449106_at 34.086105
1456379_x_at 55.55353
1434073_at 12.6989975
1417388_at 20.056631
1439610_at 30.873573
1415904_at 29.269447
1419463_at 20.188515
1421840_at 45.501625
1420388_at 29.17151
1433909_at 24.728283
1432466_a_at 11.672317
1422596_at 23.059717

Total number of rows: 45037

Table truncated, full table size 929 Kbytes.




Supplementary file Size Download File type/resource
GSM502338.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap