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Sample GSM502342 Query DataSets for GSM502342
Status Public on Jul 01, 2010
Title MEF HDAC1 fl/fl + siRNA + EtOH rep1
Sample type RNA
 
Source name MEF HDAC1 fl/fl + siRNA + EtOH
Organism Mus musculus
Characteristics cells: primary MEF
gender: Male
genotype: HDAC2 Kd
Treatment protocol cells were treatment with either Ethanol or Tamoxifen at 200 nanoMolar
Growth protocol MEF cultures were grown at 37°C in Dulbecco’s-modified Eagle’s medium supplemented with 10% heat-inactivated FBS and split 1:3.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Minikit (Qiagen) according to the manufacturer’s protocol.
Label biotin
Label protocol 1-cycle IVT protocol for Affymetrix Mouse 430v2GeneChips
 
Hybridization protocol standard Affymetrix procedure
Scan protocol standard Affymetrix precedure
Description gene expression data from HDAC2 Kd cells
Data processing Affymetrix and Expressionist
 
Submission date Jan 29, 2010
Last update date Jan 29, 2010
Contact name Patrick Matthias
E-mail(s) patrick.matthias@fmi.ch
Organization name Friedrich Miescher Institute for Biomed. Research
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platform ID GPL1261
Series (1)
GSE20100 Expression data from primary MEF lacking either HDAC1, HDAC2 or both

Data table header descriptions
ID_REF
VALUE RMA normalized data

Data table
ID_REF VALUE
1448595_a_at 41.097824
1423327_at 3474.3118
1435275_at 67.44571
1449009_at 96.93627
1429159_at 27.787958
1449368_at 294.29138
1427168_a_at 19.801762
1421965_s_at 33.105995
1449106_at 36.145958
1456379_x_at 192.73055
1434073_at 15.221433
1417388_at 25.71581
1439610_at 59.105278
1415904_at 38.400574
1419463_at 44.75237
1421840_at 165.25002
1420388_at 41.083103
1433909_at 44.681145
1432466_a_at 12.756297
1422596_at 51.044342

Total number of rows: 45037

Table truncated, full table size 929 Kbytes.




Supplementary file Size Download File type/resource
GSM502342.CEL.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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