|
| Status |
Public on Jul 01, 2010 |
| Title |
MEF HDAC1 fl/fl + siRNA + Tamoxifen rep3 |
| Sample type |
RNA |
| |
|
| Source name |
MEF HDAC1 fl/fl + siRNA + Tamoxifen
|
| Organism |
Mus musculus |
| Characteristics |
cells: primary MEF
gender: Male
genotype: HDAC1 KO/HDAC2 Kd
|
| Treatment protocol |
cells were treatment with either Ethanol or Tamoxifen at 200 nanoMolar
|
| Growth protocol |
MEF cultures were grown at 37°C in Dulbecco’s-modified Eagle’s medium supplemented with 10% heat-inactivated FBS and split 1:3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with RNeasy Minikit (Qiagen) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
1-cycle IVT protocol for Affymetrix Mouse 430v2GeneChips
|
| |
|
| Hybridization protocol |
standard Affymetrix procedure
|
| Scan protocol |
standard Affymetrix precedure
|
| Description |
gene expression data from HDAC1/2 KO/Kd cells
|
| Data processing |
Affymetrix and Expressionist
|
| |
|
| Submission date |
Jan 29, 2010 |
| Last update date |
Jan 29, 2010 |
| Contact name |
Patrick Matthias |
| E-mail(s) |
patrick.matthias@fmi.ch
|
| Organization name |
Friedrich Miescher Institute for Biomed. Research
|
| Street address |
Maulbeerstrasse 66
|
| City |
Basel |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE20100 |
Expression data from primary MEF lacking either HDAC1, HDAC2 or both |
|
